Hormonal regulation of endometriotic cell growth in primary cell culture system

Summary A primary cell culture of endometriotic cells was established to examine the hormonal regulation of endometriosis. The addition of estradiol had no effect on the DNA synthesis of cultured endometriotic cells. Progesterone at 10−7 M caused a significant reduction in the amount of the DNA synthesis. An inhibitory effect of progesterone was completely eliminated by the concomitant addition of RU486, an antiprogesterone. The addition of epidermal growth factor (EGF) at 1 ng/ml significantly increased the DNA synthesis. Other growth factors, such as fibroblast growth factor and insulin-like growth factor also produced a modest but significant increase in DNA synthesis. These results seem to provide clues for clarifying the pathophysiology of endometriosis. Similar content being viewed by others References Baulieu EE (1989) RU486 — an antiprogesterone steroid with contragestive activity in women. In: Baulieu EE, Segal SJ (eds) The antiprogesterone steroid RU486 and human fertility control. Plenum Press, New York, pp 1–25 Ikeda T, Sirbasku DA (1984) Purification and properties of a mammary-uterine-pituitary tumor cell growth factor from pregnant sheep uterus. J Biol Chem 256:4049–4064 Ishihara S, Taketani Y, Mizuno M (1990) Epidermal growth factor-like immunoreactivity in human endometrium. Asia-Oceania. J Obstet Gynecol 16:165–168 Korninckx PR, Ide P, Vandenbroucke W, Brosens IA (1980) New aspects of the pathophysiology of endometriosis and associated infertility. J Reprod Med 24:257–260 Moghissi KS, Boyce CR (1976) Management of endometriosis with oral medroxyprogesterone acetate. Obstet Gynecol 47:265–267 Roddick JW, Conkey G, Jacobs EJ (1960) The hormonal response of endometriotic implants and its relationship to symptomatology. Am J Obstet Gynecol 141:1173–1177 Sirbasku DA, Kirkland WL (1976) Control of cell growth IV. Growth properties of a new cell line established from an estrogen-dependent kidney tumor of the Syrian hamster. Endocrinol 98:1260–1272 Sirbasku DA (1978) Hormone-responsive growth in vitro of a tissue culture cell line established from the MT-W9A rat mammary tumor. Cancer Res 38:1134–1165 Sonnenschein C, Weiller S, Farooki R, Soto AM (1974) Characterization of an estrogen-sensitive cell line established from normal rat endometrium. Cancer Res 34:3147–3154 Sorrentino JM, Kirkland WL, Sirbasku DA (1976) Control of cell growth I. Estrogen-dependent growth in vivo of a rat pituitary tumor cell line. J Natl Cancer Inst 560:1149–1150 Taketani Y, Mizuno M (1988) Cyclic changes in epidermal growth factor in human endometrium during menstrual cycle. Endocrinol Japon 35:19–25 Author information Authors and Affiliations Rights and permissions About this article Cite this article Taketani, Y., Mizuno, M. Hormonal regulation of endometriotic cell growth in primary cell culture system. Arch Gynecol Obstet 251, 127–132 (1992). https://doi.org/10.1007/BF02718374 Received: Accepted: Issue date: DOI: https://doi.org/10.1007/BF02718374