Autoimmune markers for non-invasive diagnosis of endometriosis in women

Menzhinskaya I.V. Menzhinskaya, I V Menzhinskaya, Melkumyan AG, A. G. Melkumyan, С. В. Павлович, Pavlovich SV, S. V. Pavlovich, Vladimir D. Chuprynin, V. D. Chuprynin, Chuprynin VD, Л. В. Ванько, Vanko LV, L V Vanko, Г. Т. Сухих, G T Sukhikh, Sukhikh GT
Biomeditsinskaia khimiia · 2020 · vol. 66(2) , pp. 162–166 · doi:10.18097/pbmc20206602162 · PMID:32420898 · W3025174338
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

This study identified IgG antibodies to tropomyosin 3, α-enolase, and estradiol as promising biomarkers for non-invasive endometriosis diagnosis, achieving high sensitivity and specificity when combined.

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The study investigated the autoantibody profile in women with stage III–IV endometriosis and assessed diagnostic performance using modified enzyme immunoassays. Among 39 patients, a broad IgG autoantibody spectrum was detected, targeting endometrial antigens (tropomyosin 3, tropomodulin 3), α-enolase, steroid hormones (estradiol, progesterone), and gonadotropic hormones, with higher detection frequency and levels for IgG to tropomyosin 3, α-enolase, estradiol, and human chorionic gonadotropin versus 26 healthy controls. IgG to tropomyosin 3, α-enolase, and estradiol showed the highest diagnostic value, and combining them yielded an AUC of 0.875 with sensitivity and specificity of 83.3% each; the main caveat is the small sample sizes and restriction to stage III–IV patients. This paper is centrally about endometriosis — specifically, autoimmune markers and their diagnostic value for non-invasive endometriosis detection.

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Abstract

Endometriosis is a common estrogen-dependent chronic disease in women of reproductive age; it is associated with dysregulation of the immune response, local inflammation, and increased formation of autoantibodies. The aim of the study was to investigate the profile of autoantibodies in women with endometriosis and to evaluate their diagnostic value using new modifications of enzyme immunoassay. In women with endometriosis of stage III-IV (n=39), a wide spectrum of autoantibodies was detected, mainly of class G, including antibodies to endometrial antigens (tropomyosin 3, tropomodulin 3), the enzyme α-enolase, steroid (estradiol, progesterone) and gonadotropic hormones. At the same time, the frequency of detection of IgG antibodies to tropomyosin 3, α-enolase, estradiol and human chorionic gonadotropin and their levels in patients with endometriosis were higher than in healthy women (n=26) (p<0.05). IgG-antibodies to tropomyosin 3, α-enolase and estradiol were characterized by higher diagnostic value for endometriosis. The diagnostic value was significantly increased when these antibodies were combined: the AUC reached 0.875 [0.772-0.978] (p<0.0001), the sensitivity and specificity were 83.3% each. Thus, autoantibodies to tropomyosin 3, α-enolase, and estradiol are promising for inclusion in the panel of biomarkers for non-invasive diagnosis of endometriosis.
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Endometriosis is a common estrogen-dependent chronic disease in women of reproductive age; it is associated with dysregulation of the immune response, local inflammation, and increased formation of autoantibodies. The aim of the study was to investigate the profile of autoantibodies in women with endometriosis and to evaluate their diagnostic value using new modifications of enzyme immunoassay. In women with endometriosis of stage III-IV (n=39), a wide spectrum of autoantibodies was detected, mainly of class G, including antibodies to endometrial antigens (tropomyosin 3, tropomodulin 3), the enzyme α-enolase, steroid (estradiol, progesterone) and gonadotropic hormones. At the same time, the frequency of detection of IgG antibodies to tropomyosin 3, α-enolase, estradiol and human chorionic gonadotropin and their levels in patients with endometriosis were higher than in healthy women (n=26) (p<0.05). IgG-antibodies to tropomyosin 3, α-enolase and estradiol were characterized by higher diagnostic value for endometriosis. The diagnostic value was significantly increased when these antibodies were combined: the AUC reached 0.875 [0.772-0.978] (p<0.0001), the sensitivity and specificity were 83.3% each. Thus, autoantibodies to tropomyosin 3, α-enolase, and estradiol are promising for inclusion in the panel of biomarkers for non-invasive diagnosis of endometriosis. Download PDF: Keywords: endometriosis, non-invasive diagnostics, autoantibodies to tropomyosin 3, α-enolase, estradiol Citation: Menzhinskaya I.V., Melkumyan A.G., Pavlovich S.V., Chuprynin V.D., Vanko L.V., Sukhikh G.T. (2020) Autoimmune markers for non-invasive diagnosis of endometriosis in women. Biomeditsinskaya Khimiya, 66(2), 162-166. Menzhinskaya I.V. et al. Autoimmune markers for non-invasive diagnosis of endometriosis in women // Biomeditsinskaya Khimiya. - 2020. - V. 66. -N 2. - P. 162-166. Menzhinskaya I.V. et al., "Autoimmune markers for non-invasive diagnosis of endometriosis in women." Biomeditsinskaya Khimiya 66.2 (2020): 162-166. Menzhinskaya, I. V., Melkumyan, A. G., Pavlovich, S. V., Chuprynin, V. D., Vanko, L. V., Sukhikh, G. T. (2020). Autoimmune markers for non-invasive diagnosis of endometriosis in women. Biomeditsinskaya Khimiya, 66(2), 162-166. Bulun S.E., Yilmaz B.D., Sison C., Miyazaki K., Bernardi L., Liu S., Kohlmeier A., Yin P., Milad M., Wei J. (2019) Endocrine Reviews, 40(4), 1048-1079. CrossRef Scholar google search Lagana A.S., Garzon S., Gotte M., Vigano P., Franchi M., Ghezzi F., Martin D.C. (2019) Int. J. Mol. Sci., 20, 5616. CrossRef Scholar google search Tosti C., Pinzauti S., Santulli P., Chapron C., Petraglia F. (2015) Reprod. Sciences, 22(9), 1053-1059. CrossRef Scholar google search Korotkova T.D., Adamyan L.V., Stepanyan A.A., Krechetova L.V., Vanko L.V. (2018) Problemy reproduktsii, 24(6), 22-31. Scholar google search Dorien F.O., Flores I., Waelkens E., D'Hooghe T. (2018) Best Practice Research Clinical Obstetrics Gynaecology, 50, 72-83. CrossRef Scholar google search Nisenblat V., Bossuyt P.M.M., Shaikh R., Farquhar C., Jordan V., Scheffers C.S., Mol B.W.J., Johnson N., Hull M.L. (2016) Cochrane Database Syst Rev., 5, CD012179. CrossRef Scholar google search Gajbhiye R., Bendigeri T., Ghuge A., Bhusane K., Begum S., Warty N., Sawant R., Padte K., Humane A., Dasmahapatra P., Chauhan A., Khan S. (2017) Reprod. Sciences, 24(3), 413-420. CrossRef Scholar google search Nabeta M., Abe Y., Kagawa L., Haraguchi R., Kito K., Ueda N., Sugita A., Yokoyama M., Kusanagi Y., Ito M. (2009) Proteomics Clin. Appl., 3, 1201-1210. CrossRef Scholar google search

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Condition tags

endometriosis

MeSH descriptors

Autoantibodies Endometriosis Autoantibodies Biomarkers Biomarkers Endometriosis Estradiol Estradiol Female Humans Phosphopyruvate Hydratase Phosphopyruvate Hydratase Tropomyosin Tropomyosin

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