Semi-quantitative analysis of HOXA11, leukemia inhibitory factor and basic transcriptional element binding protein 1 mRNA expression in the mid-secretory endometrium of patients with endometriosis

Zohreh Alizadeh, Shokrzadeh N, Naser Shokrzadeh, Saidijam M, Massoud Saidijam, Sanoee MF, Marzieh Farimani Sanoee
Iranian biomedical journal · 2011 · vol. 15(3) , pp. 66–72 · PMID:21987111 · PMC3639745 · W2134560203
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This study found significantly decreased endometrial HOXA11 and LIF mRNA expression, with a similar trend for BTEB1, in infertile patients with endometriosis compared to healthy controls.

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Abstract

BACKGROUND: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation. We also describe a semi-quantitative RT-PCR protocol optimized in our laboratory. METHODS: Eight patients with endometriosis were considered as our case and 8 fertile women as control group. Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative RT-PCR. RESULTS: We describe the detailed procedure for the analysis of HOXA11, LIF and BTEB1 mRNA levels. Endometrial HOXA11 and LIF mRNA expression levels (normalized to beta-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (P < 0.05). A similar trend was seen in BTEB1 mRNA expression. CONCLUSION: The results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis.

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Condition tags

endometriosisinfertility

MeSH descriptors

Endometriosis Endometrium Homeodomain Proteins Kruppel-Like Transcription Factors Leukemia Inhibitory Factor RNA, Messenger Base Sequence Base Sequence Case-Control Studies Case-Control Studies DNA Primers DNA Primers Electrophoresis, Agar Gel Electrophoresis, Agar Gel Endometriosis Endometriosis Endometrium Endometrium Female Female

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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