Comparison of cytokines in the peritoneal fluid and conditioned medium of adolescents and adults with and without endometriosis

Bailey AP, Amelia P. Bailey, Hill AS, Abby S. Hill, Michael T. Beste, Beste MT, Cook CD, Christi D. Cook, Vishnudas Sarda, Sarda V, Marc R. Laufer, Laufer MR, Keith B Isaacson, Isaacson KB, Keith Isaacson, Griffith LG, Linda G. Griffith, Missmer SA, Stacey A. Missmer
American journal of reproductive immunology (New York, N.Y. : 1989) · 2020 · vol. 85(3) , pp. e13347 · doi:10.1111/aji.13347 · PMID:32946598 · W3087408155
article OA: green CC0 ⤵ 3 in-corpus citations
🔓 Open OA copy Full text JSON View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-15 ⓘ

Peritoneal fluid cytokine levels differed significantly between adolescent and adult women with endometriosis, suggesting a unique pro-invasion profile in adolescents.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by qwen3.7-flash, 2026-09-02 · read from full text ⓘ

This study compared cytokine profiles in peritoneal fluid and conditioned medium from adolescents and adults with and without endometriosis to understand age-related differences in the disease's inflammatory environment. The researchers analyzed samples from patients undergoing laparoscopy, finding that while peritoneal fluid cytokines varied by age, conditioned medium from ectopic endometrial tissue showed distinct inflammatory signatures associated with the presence of lesions regardless of patient age. A major limitation noted was the small sample size for certain adolescent subgroups, which may affect the generalizability of the findings regarding younger populations. This paper is centrally about endometriosis — specifically investigating the immunological mechanisms within peritoneal fluid and tissue culture across different age groups.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

PROBLEM: To compare inflammatory- and immune-associated peritoneal cytokines of adolescents and adults with and without endometriosis. METHODS OF STUDY: In a nested case-control study in multiple university-affiliated scientific centers, ten adolescents and thirteen adults with visually and histologically confirmed endometriosis (cases), thirteen adolescents with visually suspected endometriosis but indeterminate (seven patients) or negative (six patients) histology, and fifteen adults undergoing surgery for non-malignant gynecologic disease without endometriosis (controls) underwent laparoscopic aspiration of peritoneal fluid (PF), from which PF and conditioned medium (CM) cytokine levels were assayed. RESULTS: Compared to adults with endometriosis, MCP-3, IL-12p40, MIP-1β, and IL-15 were significantly higher among adolescents with endometriosis, while TNF-β and CTACK were lower among adolescents. These differences were similar comparing adolescents with endometriosis to adult controls except for MIP-1β, which was not statistically different. MIP-1β was, however, the only cytokine observed to differ between adult cases and controls. There were no significant differences in CM cytokines among the three groups. Results were similar when analyses were restricted to samples collected (a) during menstrual cycle days 1-10, (b) from patients unexposed to exogenous hormones, or (c) from all adolescents despite presence or absence of histologic endometriosis. CONCLUSION: Biologically relevant and statistically significant differences in six PF cytokines were observed and suggest a more pro-invasion cytokine profile among adolescents with endometriosis. Adolescents with endometriosis have unique peritoneal cytokine profiles and molecular behavior when compared to adults with and without endometriosis.
Full text 1,085 characters · extracted from oa-html · click to expand
Comparison of cytokines in the peritoneal fluid and conditioned medium of adolescents and adults with and without endometriosis Name 10.1111-aji.13347.pdf Size 370.57 KB Format Adobe PDF Checksum (MD5) 9a2a556ed87c8cc47d3dea5349b9da57 Author(s) • • • • • • • • Bailey, Amelia P. Hill, Abby S. Beste, Michael T. Cook, Christi D. Sarda, Vishnudas Laufer, Marc R. Isaacson, Keith B. Griffith, Linda G. Missmer, Stacey A. Date Issued September 29, 2020 Journal American Journal of Reproductive Immunology Publisher Wiley Citation Bailey, Amelia P., Hill, Abby S., Beste, Michael T., Cook, Christi D., Sarda, Vishnudas et al. 2020. "Comparison of cytokines in the peritoneal fluid and conditioned medium of adolescents and adults with and without endometriosis." American Journal of Reproductive Immunology, 85 (3). Version Author's final manuscript MIT Department Massachusetts Institute of Technology. Department of Biological Engineering Terms of Use Creative Commons Attribution-Noncommercial-Share Alike Persistent DSpace Link DOI of Published Version https://doi.org/10.1111/aji.13347

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-html ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Cytokines Endometriosis Endometrium Inflammation Adolescent Adult Ascitic Fluid Case-Control Studies Cells, Cultured Culture Media, Conditioned Culture Media, Conditioned Cytokines Endometriosis Endometrium Female Humans Inflammation Menstrual Cycle Menstrual Cycle

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (40)

Cited by (5)

Source provenance

europepmc
last seen: 2026-10-08T06:18:23.926119+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:21:42.008780+00:00
License: CC0 · commercial use OK