Effect of GnRH-II on the ESC proliferation, apoptosis and VEGF secretion in patients with endometriosis in vitro

Huang F, Fengying Huang, Wang H, Huanping Wang, Ying Zou, Zou Y, Qiuhong Liu, Liu Q, Cao J, Jing Cao, Tuanfang Yin, Yin T
International journal of clinical and experimental pathology · 2013 · vol. 6(11) , pp. 2487–96 · PMID:24228111 · PMC3816818 · W196200635
Lab / animal OA: closed CC0 ⤵ 11 in-corpus citations
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GnRH-II inhibited proliferation, induced apoptosis, and decreased VEGF secretion in ectopic, eutopic, and normal endometrial stromal cells, with the strongest effects observed in ectopic cells.

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Abstract

OBJECTIVE: To study the effect of GnRH-II on the cell proliferation, apoptosis and secreting vascular endothelial growth factor (VEGF) of ectopic, eutopic and normal endometrial stromal cells (ESC) from patients with or without endometriosis (EMs) in vitro. METHODS: The ectopic, eutopic and normal ESC were isolated, cultured and identified, then added 0 M, 10(-10) M, 10(-8) M, 10(-6) M GnRH-II. The growth and proliferation of three ESC were measured by MTT assay; the cell apoptosis were detected with the method of Hoechst staining and Flow Cytometry test; ELISA was used to measure the VEGF concentration change by three ESC secretion. RESULTS: GnRH-II inhibited the proliferation of ectopic, eutopic ESC from patients with endometriosis and normal ESC from control patients, in a dose- and time-dependent manner (P<0.05); GnRH-II increased the apoptotic rate of three ESC in a dose-dependent manner (P<0.05); The concentration of VEGF in three ESC was significantly decreased after the treatment of GnRH-II, in a dose-dependent manner (P<0.01); And these above effects were the strongest on the ectopic than on the eutopic or normal, there were statistical significance (P0.05). CONCLUSIONS: GnRH-II significantly inhibited the cell proliferation, induced cell apoptosis and decreased the VEGF secreting of ectopic, eutopic and normal ESC in EMs in vitro, and these effects were the strongest on ectopic ESC, which suggested that GnRH-II may become a new effective treatment for endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Apoptosis Cell Proliferation Endometriosis Endometrium Gonadotropin-Releasing Hormone Stromal Cells Vascular Endothelial Growth Factor A Adult Adult Apoptosis Apoptosis Case-Control Studies Case-Control Studies Cell Proliferation Cell Proliferation Cells, Cultured Cells, Cultured Cell Shape Cell Shape Cell Shape

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