Endometriotic cyst fluid induces reactive oxygen species (ROS) in human immortalized epithelial cells derived from ovarian endometrioma

Yoichi Kobayashi, Kiyono Osanai, Kei Tanaka, Yoshiko Nishigaya, Hironori Matsumoto, Mai Momomura, Masamichi Hashiba, Shizuka Mita, Satoru Kyo, Mitsutoshi Iwashita
Redox report : communications in free radical research · 2016 · vol. 22(6) , pp. 361–366 · doi:10.1080/13510002.2016.1258448 · PMID:27866464 · PMC6837669 · W2552746135
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Endometriotic cyst fluid significantly increased reactive oxygen species (ROS) production in human ovarian endometrioma epithelial cells, indicating exposure to ECF induces strong oxidative stress.

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Abstract

OBJECTIVES: Endometriotic cyst fluid (ECF) contains a large amount of reactive oxygen species (ROS), and endometriotic cysts are exposed to strong oxidative stress, which may cause malignant transformation. In this study, ROS production by ECF was clinically analysed. METHODS: Human immortalized epithelial cells derived from ovarian endometrioma (EMosis-CC/TERT 1) were treated with ECF. In addition, ROS production in EMosis-CC/TERT 1 was measured, and its clinical significance was analysed. RESULTS: A total of 38 ECF samples were obtained from patients diagnosed with endometriotic cysts. In EMosis-CC/TERT1, significantly higher levels of ROS were induced by ECF than by the vehicle control and ferric nitrilotriacetate. There were no significant differences in ROS production by laterality and preoperative serum CA125 values. There were several patients whose cyst sizes were approximately 5 cm and had relatively high ROS production. Production of ROS by ECF was relatively higher in patients older than 40 years of age than in those younger than 40. DISCUSSION: Our study revealed that ROS are highly produced by ECF in EMosis-CC/TERT1 cells; therefore, exposure to ECF induced strong oxidative stress. Development of a therapeutic strategy to reduce ROS production might be useful for preventing malignant transformation of endometriotic cysts.

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Condition tags

endometriosisendometrioma

MeSH descriptors

Cyst Fluid Endometriosis Epithelial Cells Epithelial Cells Reactive Oxygen Species Adult CA-125 Antigen CA-125 Antigen Cell Line Cell Transformation, Neoplastic Cyst Fluid Endometriosis Endometriosis Epithelial Cells Female Humans Membrane Proteins Membrane Proteins Oxidative Stress Oxidative Stress

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