DETERMINATION OF ANTIGEN LEVELS: T-PLASTIN, TRANSGELIN AND CA-125 IN BABOONS WITH INDUCED ENDOMETRIOSIS

Ann W. Githinji, Kenneth Ogila, Mutinda C. Kyama, Cleophas Kyama, Vicky Gent, Erastus Mwadondo, Nyachieo Atunga, Daniel Chai, Rebecca Waihenya
In: Advances in Life Science and Technology · 2019 · doi:10.7176/alst/75-06 · W2970278996
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study measured T-plastin, Transgelin, and CA-125 in baboons with induced endometriosis, finding CA-125 in peritoneal fluid to be a potential diagnostic biomarker.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 ⓘ

This prospective case-control study used ten proven-fertile female baboons with induced endometriosis (intra-pelvic injection of menstrual endometrium on day 1–2, followed by staging laparoscopy) and compared peripheral blood and peritoneal fluid levels of T-plastin, transgelin, and CA-125 before and after induction. T-plastin and transgelin were measured by ELISA, and statistical comparisons showed insignificant changes in both compartments for these two antigens, while CA-125 showed significant differences (p=0.0003 in peripheral blood and p=0.0279 in peritoneal fluid). Receiver operating characteristic analyses yielded AUC=0.5 for T-plastin and transgelin, indicating unreliable diagnostic performance, whereas CA-125 in peritoneal fluid had an AUC of 0.7900. The paper directly evaluates biomarker candidacy in a baboon model but its diagnostic interpretation is constrained to early detection performance in this experimental setting. This paper is centrally about endometriosis—assessing T-plastin, transgelin, and CA-125 as potential early diagnostic biomarkers in a baboon model of induced endometriosis.

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Abstract

Endometriosis is defined as the presence of endometrial-like tissue outside the uterine cavity and is associated with chronic intra-pelvic inflammation. Its symptoms, which are often severe, have a negative impact on a woman’s quality of life. They include chronic pelvic pain and infertility. It is estimated that about 10% women in their reproductive ages are affected by the disease globally. Currently, diagnostic methods are laparoscopy with histological confirmation and non-invasive such as ultrasound and the quantification of CA-125 biomarker in serum. CA-125 is known to be elevated in endometriosis, however it does not have sufficient diagnostic power as a single biomarker of endometriosis. Due to this, there is a need to determine other antigens that would act as potential biomarkers for endometriosis. It has been observed that Transgelin and T-Plastin are upregulated in endometriosis lesions and in secretory phase of endometrium respectively. There is insufficient data available showing the concentration of T-plastin in serum or plasma in the development of endometriosis. The main objective of this study was to identify whether T-plastin, Transgelin and CA-125 are potential biomarkers in early diagnosis of endometriosis using animal model for endometriosis. The baboon model represents clinically relevant research models for endometriosis. This study compared the levels of T-plastin, Transgelin and CA-125 in the peripheral blood and peritoneal fluid of baboons before and after induction of endometriosis. In this prospective, case-control study, ten female baboons ( Papio anubis , 9-15 kg each) of proven fertility that have had at least one menstruation during captivity, were induced by intra-pelvic injection of menstrual endometrium on day 1 or 2 of menstruation followed by staging laparoscopy. Serum and peritoneal fluid samples were collected prior to induction to serve as controls.T-Plastin, Transgelin and CA-125 were measured using commercially available ELISA Kits. Data was analyzed using non-parametric test and level of significance at p<0.005. T-Plastin and Transgelin had insignificant p values in both peripheral blood and peritoneal fluid while CA-125 showed p=0.0003 in peripheral blood and p=0.0279 in peritoneal fluid. Diagnostic performance of individual biomarkers was determined by Receiver Operating Characteristic (ROC) with the Area under the curve (AUC) equal to 0.5 showing T-Plastin and Transgelin in both serum and peritoneal fluid unreliable for use as biomarkers in endometriosis. CA-125 levels in peritoneal fluid had an AUC of 0.7900 indicating it can be used as potential biomarkers for endometriosis. Key words: Endometriosis, biomarkers, T-Plastin, Transgelin, CA-125, ELISA, Olive Baboons. DOI : 10.7176/ALST/75-06 Publication date :August 31 st 2019

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endometriosischronic_pelvic_paininfertility

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