Methylation analysis of plasminogen activator inhibitor-1 (PAI-1) gene in ovarian and peritoneal endometriosis

Tutug Kinasih, Ririn Rahmala Febri, Nagita Gianty Annisa, Raden Muharam Natadisastra, Asmarinah Asmarinah
In: Journal of Physics: Conference Series · 2018 · vol. 1073 , pp. 032081 · doi:10.1088/1742-6596/1073/3/032081 · W2889838642
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This study found lower PAI-1 gene methylation levels in ovarian and peritoneal endometriosis tissues compared to controls, suggesting increased gene expression contributes to endometriosis risk.

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Abstract

Endometriosis, defined as the growth of endometrial-like tissue outside the uterus, leads to the emergence of chronic inflammatory reactions, which are influenced by the plasminogen activator systems that are known to play a role in fibrinolysis. Hypofibrinolysis due to the excessive expression of the plasminogen activator inhibitor-1 (PAI-1) gene occurs in endometriotic cells. It has been known that the PAI-1 level is lower in normal endometrial cells than in endometriotic cells. The aim of this study is to assess the methylation level of PAI-1 in association with ovarian and peritoneal endometriosis tissues. This was a comparative cross-sectional study conducted on 13 women with ovarian endometriosis, 5 with peritoneal endometriosis, and 8 without endometriosis. DNA from the patient samples was isolated and subjected to bisulfite conversion. DNA methylation was observed by performing the methylation-specific polymerase chain reaction (MSP) method, followed by electrophoresis. The methylation level of PAI-1 was determined by measuring the band intensity using the ImageJ software. The results were statistically analyzed using the Kruskal–Wallis test. A twotailed p value of <0.05 was considered to be statistically significant. Statistically significant difference was noted in the methylation levels of PAI-1 in ovarian endometriosis and peritoneal endometriosis samples compared with those noted in control samples (p = 0.006 and p = 0.003, respectively). The methylation levels in both sample types were lower than those in the control samples. However, the difference between the methylation levels of PAI-1 in peritoneal endometriosis and ovarian endometriosis were not statistically significant (p > 0.05). We found a low methylation level in the promoter region of PAI-1 gene, which led to an increase in the gene expression that may contribute as a risk factor in ovarian endometriosis and peritoneum endometriosis.

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endometriosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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