Methylation analysis of plasminogen activator inhibitor-1 (PAI-1) gene in ovarian and peritoneal endometriosis

Tutug Kinasih, Ririn Rahmala Febri, Nagita Gianty Annisa, Raden Muharam Natadisastra, Asmarinah Asmarinah
In: Journal of Physics: Conference Series · 2018 · vol. 1073 , pp. 032081 · doi:10.1088/1742-6596/1073/3/032081 · W2889838642
article OA: diamond CC0
🔓 Open OA copy View on OpenAlex View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This study found lower PAI-1 gene methylation levels in ovarian and peritoneal endometriosis tissues compared to controls, suggesting increased gene expression contributes to endometriosis risk.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Endometriosis, defined as the growth of endometrial-like tissue outside the uterus, leads to the emergence of chronic inflammatory reactions, which are influenced by the plasminogen activator systems that are known to play a role in fibrinolysis. Hypofibrinolysis due to the excessive expression of the plasminogen activator inhibitor-1 (PAI-1) gene occurs in endometriotic cells. It has been known that the PAI-1 level is lower in normal endometrial cells than in endometriotic cells. The aim of this study is to assess the methylation level of PAI-1 in association with ovarian and peritoneal endometriosis tissues. This was a comparative cross-sectional study conducted on 13 women with ovarian endometriosis, 5 with peritoneal endometriosis, and 8 without endometriosis. DNA from the patient samples was isolated and subjected to bisulfite conversion. DNA methylation was observed by performing the methylation-specific polymerase chain reaction (MSP) method, followed by electrophoresis. The methylation level of PAI-1 was determined by measuring the band intensity using the ImageJ software. The results were statistically analyzed using the Kruskal–Wallis test. A twotailed p value of <0.05 was considered to be statistically significant. Statistically significant difference was noted in the methylation levels of PAI-1 in ovarian endometriosis and peritoneal endometriosis samples compared with those noted in control samples (p = 0.006 and p = 0.003, respectively). The methylation levels in both sample types were lower than those in the control samples. However, the difference between the methylation levels of PAI-1 in peritoneal endometriosis and ovarian endometriosis were not statistically significant (p > 0.05). We found a low methylation level in the promoter region of PAI-1 gene, which led to an increase in the gene expression that may contribute as a risk factor in ovarian endometriosis and peritoneum endometriosis.

My notes (saved in your browser only)

Condition tags

endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (22)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK