Knockdown hsa_circ_0063526 inhibits endometriosis progression via regulating the miR-141-5p / EMT axis and downregulating estrogen receptors

Zhangming Wei, Yi Hu, Xiang He, Mengmeng Zhang, Xinyue Zhang, Yali Wang, Xiaoling Fang, Liping Li
Aging · 2021 · vol. 13(24) , pp. 26095–26117 · doi:10.18632/aging.203799 · PMID:34967761 · W4200026744
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This study found that knockdown of hsa_circ_0063526 inhibited endometriosis progression by regulating the miR-141-5p/EMT axis and downregulating estrogen receptors, suggesting it as a potential therapeutic target.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This paper investigates whether the circular RNA hsa_circ_0063526 (circ-RanGAP1) influences endometriosis progression by modulating miR-141-5p and the EMT pathway, and it also examines downstream estrogen receptor regulation. Using RT-qPCR in 41 patient lesion-versus-control samples, the authors report higher hsa_circ_0063526 and lower miR-141-5p in endometriosis, with a negative correlation between them, supported by a luciferase reporter showing direct binding of miR-141-5p to hsa_circ_0063526; the main limitation is that the functional work appears confined to End1/E6E7 cell experiments rather than in vivo models or tissue-level mechanistic validation. In End1/E6E7 cells, knockdown of hsa_circ_0063526 via siRNA decreases proliferation, invasion, and migration, increases E-cadherin (an epithelial EMT marker), and effects can be reversed by inhibiting miR-141-5p, consistent with regulation along an miR-141-5p/EMT axis. This paper is centrally about endometriosis — it studies circ-RanGAP1 (hsa_circ_0063526) knockdown and its mechanistic link to miR-141-5p and EMT-related changes in endometriosis cells.

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Abstract

Endometriosis can cause severe social burdens. Abnormal circular RNA levels have been found to lead to changes of related gene expression, thereby mediating the occurrence and development of a series of diseases, including endometriosis. The role of circRNA in endometriosis is still in its infancy. This study will explore the role of circRNA hsa_circ_0063526 with microRNA-141-5p in the development of endometriosis. The expression levels of genes were detected by RT-qPCR. Transwell, wound-healing, and EdU assays were performed on the End1 / E6E7 cell line from the endometriosis patient. PCR and immunohistochemistry were used to detect the expression of candidate regulatory genes in ectopic lesions in an endometriosis mice model. The expression level of hsa_circ_0063526 in ectopic tissue of endometriosis patients was significantly higher than control (P<0.05), The expression levels of hsa_circ_0063526 and miRNA-141-5P in ectopic tissue of endometriosis were negatively correlated (P<0.05). Knockdown of hsa_circ_0063526 inhibited the invasion, migration, and proliferation ability of End1 / E6E7 cell; the inhibition of microRNA-141-5p rescued this inhibition (P <0.05). In vivo experiments showed that miR-141-5p and si-hsa_circ_0063526 treatment reduced lesion size and regulated endometriosis genes. Our data suggest that hsa_circ_0063526 and miR-141-5p are possible biomarkers and therapeutic targets for endometriosis.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Down-Regulation Endometriosis Gene Expression Regulation Gene Knockdown Techniques Receptors, Estrogen RNA, Circular Animals Endometriosis Female Humans Mice MicroRNAs MicroRNAs MicroRNAs Receptors, Estrogen

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