Thalidomide Inhibits Tumor Necrosis Factor-α-Induced Interleukin-8 Expression in Endometriotic Stromal Cells, Possibly through Suppression of Nuclear Factor-κB Activation

Tatsuo Yagyu, Hiroshi Kobayashi, Hidenori Matsuzaki, H Matsuzaki, Kiyoshi Wakahara, Toshiharu Kondo, Noriyuki Kurita, Hideo Sekino, Kiyokazu Inagaki, Mika Suzuki, Naohiro Kanayama, Toshihiko Terao
The Journal of clinical endocrinology and metabolism · 2005 · vol. 90(5) , pp. 3017–3021 · doi:10.1210/jc.2004-1946 · PMID:15687330 · W2140855867
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Thalidomide treatment attenuated tumor necrosis factor-alpha-induced interleukin-8 expression and nuclear factor-kappaB activation in human endometriotic stromal cells.

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Abstract

OBJECTIVE: Endometriosis, a common disease among women of reproductive age, is characterized by the presence of endometrial-like tissue outside the uterus. TNF-alpha induces IL-8 production in endometriotic cells through nuclear factor-kappaB (NF-kappaB) activation. Thalidomide (Thal) inhibits inflammation by down-regulating the expression of proinflammatory cytokines in tumor cells and inflammatory cells. However, the mechanism of Thal action in human endometriotic stromal cells has not yet been elucidated. MAIN OUTCOME MEASURES: We examined whether Thal abrogates TNF-alpha-induced up-regulation of IL-8 expression in endometriotic stromal cells. RESULTS: Here, we show 1) that treatment of endometriotic stromal cells with TNF-alpha increased the expression of phosphorylated IkappaBalpha and degradation of total IkappaBalpha, which in turn activates NF-kappaB; 2) Thal significantly inhibits the TNF-alpha-induced expression of phosphorylated IkappaBalpha and degradation of IkappaBalpha; 3) TNF-alpha activation induced increased nuclear translocation of NF-kappaB, which was inhibited by pretreatment with either Thal or N-tosyl-L-phenylalanine chloromethyl ketone, an NF-kappaB inhibitor. Thal did not enhance the N-tosyl-L-phenylalanine chloromethyl ketone's action; and 4) Pretreatment with Thal reduced TNF-alpha-induced IL-8 protein production as well as mRNA expression. CONCLUSION: The current study showed for the first time that Thal treatment attenuated the expression of IL-8 by reducing TNF-alpha-induced NF-kappaB activation.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis I-kappa B Proteins Interleukin-8 Thalidomide Tumor Necrosis Factor-alpha Active Transport, Cell Nucleus Cells, Cultured Endometriosis Female Humans I-kappa B Proteins Interleukin-8 Interleukin-8 NF-KappaB Inhibitor alpha Stromal Cells Stromal Cells Thalidomide Tosylphenylalanyl Chloromethyl Ketone Tosylphenylalanyl Chloromethyl Ketone Tumor Necrosis Factor-alpha

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