Increased Migration Ability of Adenomyosis-Derived Endometrial Mesenchymal Stem Cells

Adenomyosis is a form of endometriosis—a gynecological disease associated with abnormal functional activity of endometrial cells. Endometrial stem cells can play a key role in the pathogenesis of this disease. Despite the numerous studies that have been conducted of cultures of endometrial mesenchymal stem cells (eMSCs) obtained from patients with adenomyosis, information on their phenotypic and functional properties is very contradictory. In this work, a comparative study of morphological and migratory characteristics of human endometrial mesenchymal stem cells isolated from the menstrual blood of healthy donors (eMSCs) and a donor with adenomyosis (eMSCs-A) was performed. eMSC migration was evaluated by the method of “wound healing” using live-cell microscopy. It was established that the rate of wound healing of eMSCs-A is significantly higher compared to normal cells, which indicates an increased migration potential of eMSCs-A in adenomyosis. However, when transferring cells to a serum-free medium, eMSC-A migrated more slowly than normal cells. As a result of the assessment of morphological characteristics, it was found that eMSCs-A are smaller (in area and perimeter) than normal eMSCs, while the remaining morphometric parameters reflecting cell polarization did not differ. The data obtained allow the use of eMSCs in culture as a model for elucidating the membrane and intracellular mechanisms that underlie changes in cellular mechanics, motility, and invasive activity in various pathologies, including adenomyosis. Similar content being viewed by others

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Funding This study was financed by the Russian Science Foundation, project no. 18-15-00106. Author information Authors and Affiliations Corresponding author Ethics declarations The authors declare that they have no conflict of interest.The authors did not conduct experiments involving animals or human beings. Cell lines obtained and characterized previously (Shilina et al., 2015) were used. Additional information Abbreviations: eMSC—endometrial mesenchymal stem cell isolated from the menstrual blood of healthy donors, eMSC-A—eMSC isolated from menstrual blood of a donor with adenomyosis, FBS—fetal bovine serum. Rights and permissions About this article Cite this article Sudarikova, A.V., Shilina, M.A., Chubinskiy-Nadezhdin, V.I. et al. Increased Migration Ability of Adenomyosis-Derived Endometrial Mesenchymal Stem Cells. Cell Tiss. Biol. 14, 190–195 (2020). https://doi.org/10.1134/S1990519X20030062 Received: Revised: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1134/S1990519X20030062