Curcumin improves growth factors expression of bovine cumulus-oocyte complexes cultured in peritoneal fluid of women with endometriosis

Hendarto H, Hendy Hendarto, Muhammad Yohanes Ardianta Widyanugraha, Yohanes Ardianta Widyanugraha M, Widjiati Widjiati
International journal of reproductive biomedicine · 2019 · vol. 16(12) , pp. 775–782 · doi:10.18502/ijrm.v16i12.3683 · PMID:31417983 · PMC6600283 · W2920311875
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Curcumin addition to peritoneal fluid from women with endometriosis improved growth factor expression, specifically increasing GDF-9 and KitL while affecting TNFα, in cultured bovine cumulus-oocyte complexes.

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This laboratory experimental study cultured bovine cumulus–oocyte complexes in tissue culture medium alone (control), or supplemented with peritoneal fluid from infertile women with endometriosis, with or without added curcumin, then used immunohistochemistry to assess the semi-quantitative expression of GDF-9, Kit ligand, and TNF-α. The peritoneal fluid from infertile women with endometriosis reduced GDF-9 and KitL expression compared with control, while TNF-α increased; adding curcumin partially reversed these effects by increasing GDF-9 and KitL and decreasing TNF-α relative to the peritoneal-fluid-only condition. A key limitation is that only 21 COCs (7 per group) were analyzed and the staining-based assessment is semi-quantitative rather than direct functional outcome measurement. This paper is centrally about endometriosis — it tests whether curcumin can modulate oocyte–granulosa growth factor and inflammatory signaling in bovine COCs exposed to endometriosis-associated peritoneal fluid.

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Abstract

Background: Peritoneal fluid (PF) from infertile women with endometriosis contains inflammatory mediators that may interfere with folliculogenesis. Objective: The aim was to evaluate the effects of curcumin on growth factors expression by evaluating Growth Differentiation Factor-9 (GDF-9), Kit Ligand (KitL), and Tumor Necrosis Factor-9 (GDF-9), Kit Ligand (KitL), and Tumor Necrosis Factor (COC)s cultured with PF from infertile women with endometriosis. Materials and Methods: In this experimental study, 21 infertile women (aged between 20 and 40 years) who refered to Dr Sutomo Hospital from January to July 2015 were enrolled. COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong anti-inflammatory turmeric, was added in Tissue Culture Medium 199 (TCM199) and PF for culture medium. Bovine COCs were cultured into three groups of the medium: 1. TCM199, 2. TCM199 + PF, and 3. TCM199 + PF + curcumin. GDF9, KitL, and TNFα expressions were examined using immunohistochemistry technique. Results: GDF-9 expression of bovine COCs cultured in PF with curcumin addition (2.67 ± 0.98) was found to increase compared to those cultured without curcumin (0.50 ± 0.67) (p ≤ 0.001). It was similar to KitL expression of bovine COCs cultured with curcumin (2.67 ± 1.23), which increased compared to those without curcumin (0.33 ± 0.49) (p ≤ 0.001). A significant difference in TNFα expression was noted between groups with or without curcumin (p ≤ 0.001). Conclusion: In the culture of PF from infertile women with endometriosis, curcumin addition improves the growth factors expression of bovine COCs. The increase of GDF9 and KitL expressions will improve folliculogenesis. Key words: Curcumin, Bovine, GDF9, Kit Ligand, TNF.
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Our findings suggest that in the culture of PF from infertile women with endometriosis, curcumin addition improved growth factors expression of bovine COCs through the decrease of inflammation. The increase of GDF-9 and KitL expressions due to the anti-inflammatory effect of curcumin will improve oocyte–granulosa cell interaction and subsequently folliculogenesis. Further studies are warranted.

Coi Statement

The authors report no conflict of interest.

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endometriosisinfertility

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