Curcumin Improves the Regulation of Ovarian Folliculogenesis in Culture Media with Peritoneal Fluid from Infertile Women with Endometriosis

Hendy Hendarto
Indian Journal of Forensic Medicine & Toxicology · 2020 · doi:10.37506/ijfmt.v14i2.3154 · W4242281838
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Curcumin addition to bovine cumulus oocyte complexes cultured with endometriosis patient peritoneal fluid improved growth differentiation factor-9 and Kit ligand expression while decreasing tumor necrosis factor-alpha.

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The study evaluated whether curcumin modulates ovarian folliculogenesis-related factors when bovine cumulus oocyte complexes (COCs) are cultured in the presence of peritoneal fluid (PF) from infertile women with endometriosis. Using a three-group culture design (TCM199 alone, TCM199 plus endometriosis PF, and TCM199 plus endometriosis PF with curcumin), the authors assessed GDF-9, Kit ligand (KitL), and TNF-α expression by immunohistochemistry. Curcumin increased GDF-9 and KitL expression compared with culture with endometriosis PF without curcumin, and it also produced a significant difference in TNF-α expression across groups, consistent with reduced inflammatory signaling; however, the measured GDF-9 increase with curcumin did not match the control condition, indicating incomplete normalization. This paper is centrally about endometriosis — specifically, it tests curcumin’s effects on ovarian folliculogenesis regulation using endometriosis patient peritoneal fluid in a bovine COC culture model.

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Abstract

Background : Peritoneal fluid (PF) from infertile women with endometriosis contains a variety of inflammatory mediators that may interfere with folliculogenesis. The aim of the study was to evaluate effects of curcumin on the regulation of ovarian folliculogenesis by evaluating Growth Differentiation Factor (GDF)-9, Kit Ligand (KitL) and Tumor Necrosis Factor (TNF)? expressions in bovine cumulus oocyte complexes (COC)s cultured with PF from infertile women with endometriosis. Method: COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong antiinflammatory turmeric, was added in Tissue Culture Media (TCM)199 and PF from infertile women with endometriosis for culture media. Bovine COCs were cultured into 3 groups of media: 1) TCM199, 2) TCM199+PF from infertile women with endometriosis, and 3) TCM199+PF from infertile women with endometriosis+curcumin. GDF-9, KitL and TNF? expressions were examined using immunohistochemistry technique. Results: GDF-9 expression of bovine COCs cultured in PF from infertile women with endometriosis with curcumin addition (2.67±0.98) was found to increase compared to those cultured without curcumin (0.50±0.67) but reduced compared to the control. This result was similar to KitL expression of bovine COCs cultured with curcumin (2.67±1.23), in which it increased compared to those without curcumin (0.33±0.49) (p<0.05). A significant difference in TNF? expression was noted between groups with or without curcumin (p<0.05). Conclusion: In the culture of PF from infertile women with endometriosis, curcumin addition may improve the regulation of ovarian folliculogenesis through the decrease of inflammation factor.
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Curcumin Improves the Regulation of Ovarian Folliculogenesis in Culture Media with Peritoneal Fluid from Infertile Women with Endometriosis DOI: https://doi.org/10.37506/ijfmt.v14i2.3154Keywords: endometriosis; curcumin; GDF9; kit ligand; TNF?Abstract Background : Peritoneal fluid (PF) from infertile women with endometriosis contains a variety of inflammatory mediators that may interfere with folliculogenesis. The aim of the study was to evaluate effects of curcumin on the regulation of ovarian folliculogenesis by evaluating Growth Differentiation Factor (GDF)-9, Kit Ligand (KitL) and Tumor Necrosis Factor (TNF)? expressions in bovine cumulus oocyte complexes (COC)s cultured with PF from infertile women with endometriosis. Method: COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong antiinflammatory turmeric, was added in Tissue Culture Media (TCM)199 and PF from infertile women with endometriosis for culture media. Bovine COCs were cultured into 3 groups of media: 1) TCM199, 2) TCM199+PF from infertile women with endometriosis, and 3) TCM199+PF from infertile women with endometriosis+curcumin. GDF-9, KitL and TNF? expressions were examined using immunohistochemistry technique. Results: GDF-9 expression of bovine COCs cultured in PF from infertile women with endometriosis with curcumin addition (2.67±0.98) was found to increase compared to those cultured without curcumin (0.50±0.67) but reduced compared to the control. This result was similar to KitL expression of bovine COCs cultured with curcumin (2.67±1.23), in which it increased compared to those without curcumin (0.33±0.49) (p<0.05). A significant difference in TNF? expression was noted between groups with or without curcumin (p<0.05). Conclusion: In the culture of PF from infertile women with endometriosis, curcumin addition may improve the regulation of ovarian folliculogenesis through the decrease of inflammation factor. Downloads Published Issue Section License https://creativecommons.org/licenses/by-nc/2.0/deed.en

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