TNFalpha gene silencing reduced lipopolysaccharide-promoted proliferation of endometriotic stromal cells

Miyamoto A, Ayako Miyamoto, Fuminori Taniguchi, Taniguchi F, Yukiko Tagashira, Tagashira Y, Ayako Watanabe, Watanabe A, Harada T, Tasuku Harada, Terakawa N, Naoki Terakawa
American journal of reproductive immunology (New York, N.Y. : 1989) · 2009 · vol. 61(4) , pp. 277–285 · doi:10.1111/j.1600-0897.2009.00691.x · PMID:19260858 · W1569502812
Lab / animal OA: closed CC0 ⤵ 8 in-corpus citations
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TNFα gene silencing repressed LPS-induced cIAP2 and IL-8 expression and attenuated LPS-promoted endometriotic stromal cell proliferation.

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Abstract

PROBLEM: We previously reported that lipopolysaccharide (LPS)-promoted endometriotic stromal cell (ESC) proliferation by inducing TNFalpha production. The aim of this study was to investigate the efficacy of TNFalpha gene silencing on LPS-treated ESCs. METHOD OF STUDY: Endometriotic stromal cells (ESCs) and endometrial stromal cells (ESCs) (EMSCs) were obtained from ovarian chocolate cysts and uterine myoma, respectively. Using PCR array, LPS-induced gene expression profiling after transfection of TNFalpha siRNA into ESCs was performed. Down-regulated genes by TNFalpha silencing were examined using real-time RT-PCR. Effect of TNFalpha silencing was examined using ELISA and BrdU incorporation, respectively. RESULTS: In PCR array, TNFalpha silencing in ESCs repressed LPS-induced expression of cIAP2 and IL-8, NFkappaB pathway responsive genes. After adding LPS, the levels of cIAP2 and IL-8 expression in ESCs were higher compared with those in EMSCs. TNFalpha silencing attenuated the LPS-induced ESC proliferation. CONCLUSION: Tumor necrosis factor alpha may be involved in cell proliferation of endometriotic tissues.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Leiomyoma Ovarian Cysts Ovarian Diseases Tumor Necrosis Factor-alpha Uterine Neoplasms Apoptosis Apoptosis Apoptosis Baculoviral IAP Repeat-Containing 3 Protein Baculoviral IAP Repeat-Containing 3 Protein Cell Proliferation Cell Proliferation Cell Proliferation Cells, Cultured Cells, Cultured Endometriosis Endometriosis Endometriosis Endometriosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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