Determination of Danazol in Plasma by High Performance Liquid Chromatography
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This paper describes an HPLC method using an ODS column and UV-Vis detection for quantifying danazol in plasma after acetonitrile extraction and cadmium sulfate pretreatment.
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Abstract
Abstract An HPLC method has been developed for the determination of danazol in human plasma. the chromatographic conditions consisted of an ODS column 80 × 7.0 mm, 3 μm; a mobile phase of 71:29, methanol:20 mM potassium dihydrogen phosphate. A UV-Visible detector was set at 288 nm to monitor the danazol peak. Danazol was extracted from plasma with acetonitrile which was salted out with potassium carbonate. Prior to salting out, cadmium sulfate was mixed with the acetonitrile-plasma mixture to remove any interfering constituents. the extracted acetonitrile layer was evaporated and the residue was reconstituted in the mobile phase before injection. the method was found to be reproducible with relative standard deviation (%rsd) from 2.3 to 7.2%. A number of clinically important drugs did not interfere with danazol determination.
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Cited by (8)
- Development and Validation of Stability Indicating HPLC Method for Estimation of Anti-Gonadotropic Drug Danazol in Bulk and Capsule: Degradation Study Characterization of Alkali Degradation Product and ADME/Tox Evaluation 2025
- Danazol 2022
- New Extractive Spectrophotometric Method Development and Validation for the Estimation of Danazol in Pharmaceutical Formulations 2019
- Prediction of positive food effect: Bioavailability enhancement of BCS class II drugs 2016
- Square-wave adsorptive stripping voltammetric determination of danazol in capsules 2006
- Voltammetric study of danazol and its determination in capsules and spiked biological fluids 2004
- Conventional and micellar liquid chromatography method development for danazol and validation in capsules 2003
- Photodegradation kinetic study and stability-indicating assay of danazol using high-performance liquid chromatography 2000
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- openalex
- last seen: 2026-05-10T10:44:01.662386+00:00
- unpaywall
- last seen: 2026-06-21T16:06:39.831647+00:00
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