Quantitative DNA perturbations of p53 in endometriosis: analysis of American and Icelandic cases

Jón Torfi Gylfason, Jon Torfi Gylfason, Gylfason JT, Dianne Dang, Dang D, Vigdis Petursdottir, Vigdís Pétursdóttir, Petursdottir V, Kristrún R. Benediktsdóttir, Kristrun R Benediktsdottir, Benediktsdottir KR, Reynir T Geirsson, Reynir Tómas Geirsson, Geirsson RT, Alfred N. Poindexter, Alfred Poindexter, Poindexter A, Dorothy Mitchell‐Leef, Dorothy Mitchell-Leef, Mitchell-Leef D, John E Buster, Buster JE, Carson SA, Sandra A Carson, Sandra Ann Carson, Joe Leigh Simpson, Farideh Z Bischoff, Bischoff FZ
Fertility and sterility · 2005 · vol. 84(5) , pp. 1388–1394 · doi:10.1016/j.fertnstert.2005.05.031 · PMID:16275233 · W1969458733
article OA: closed CC0 ⤵ 4 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-22 ⓘ

This study analyzed quantitative DNA alterations of the p53 tumor suppressor gene in endometriosis patient samples from American and Icelandic cohorts.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

ObjectiveTo investigate quantitative aberrations involving p53 copy numbers in eutopic endometrial and endometriotic tissue from two populations.DesignComparative analysis of normal and diseased tissue.SettingTissue specimens collected in Iceland and USA.Patient(s)Subjects with moderate/severe endometriosis (Iceland, n = 26; USA, n = 45). Paraffin-embedded tissue from 19 matched Icelandic cases and seven unaffected controls. American cases were fresh surgical tissue from 17 matched cases and 28 unaffected controls. DNA isolation and real-time polymerase chain reaction (PCR) with TaqMan assay were performed.Main outcome measure(s)The frequency of p53 loss and/or gain based on quantitative differences for copy numbers of p53 located on chromosome (17p) and GAPDH on a control locus (chromosome 12p).Result(s)Among American cases, significant p53 gain (n = 13) or loss (n = 4) was observed in 17 of 21 cases. In Icelandic cases this was not seen to the same degree. Mean normalized p53 values were 3.46 and 1.16 copies per reaction, respectively. Significant differences were observed between normalized p53 in the control blood and affected tissue for the American and Icelandic cases compared to standard GAPDH control but not in normal Icelandic and American endometrium.Conclusion(s)The results continue to support a role for nonrandom somatic p53 locus alterations in the pathogenesis of late or severe-stage endometriosis. Differences between Icelandic and American subjects have implications for generalization of genome-wide approaches.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

DNA Endometriosis Genes, p53 Quantitative Trait Loci DNA Endometriosis Female Genes, p53 Humans Iceland Quantitative Trait Loci United States

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (56)

Cited by (4)

Source provenance

europepmc
last seen: 2026-07-29T06:27:48.050232+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:15:29.922408+00:00
unpaywall
last seen: 2026-07-29T07:08:50.896093+00:00
License: CC0 · commercial use OK