Elucidating the role of long intergenic non-coding RNA 339 in human endometrium and endometriosis

Sarah J Holdsworth-Carson, Sarah J. Holdsworth‐Carson, Molly Churchill, Churchill M, Jacqueline F Donoghue, Sally Mortlock, Jenny N Fung, Clare Sloggett, Jessica Chung, Cann L, Leonie Cann, Wan Tinn Teh, Teh WT, Campbell KR, Katie-Rose Campbell, Rodney Luwor, Rodney B. Luwor, Luwor R, Healey M, Martin Healey, Grant W. Montgomery, Grant Montgomery, Montgomery G, Jane E Girling, Peter A W Rogers
Molecular human reproduction · 2021 · vol. 27(3) · doi:10.1093/molehr/gaab010 · PMID:33576410 · W3126423828
article OA: closed CC0 ⤵ 6 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-23 ⓘ

This study characterized LINC00339 expression in human endometrium and endometriosis lesions, revealing its restriction to lesion foci and its role in regulating immune defense pathways in endometrial cells.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Endometriosis is a complex disease, influenced by genetic factors. Genetic markers associated with endometriosis exist at chromosome 1p36.12 and lead to altered expression of the long intergenic non-coding RNA 339 (LINC00339), however, the role of LINC00339 in endometriosis pathophysiology remains unknown. The aim of this work was to characterize the expression patterns of LINC00339 mRNA in endometrium and endometriotic lesions in situ and to determine the functional role of LINC00339 in human endometrium. We employed RNA-sequencing (RNA-seq), quantitative RT-PCR and in situ hybridization to investigate the abundance of LINC00339 transcripts in endometrium and endometrial cell lines and to describe the pattern and localization of LINC00339 expression in endometrium and endometriotic lesions. LINC00339 mRNA expression was manipulated (overexpressed and silenced) in endometrial stromal cell lines and RNA-seq data from overexpression models were analysed using online bioinformatics platforms (STRING and Ingenuity Pathway Analysis) to determine functional processes. We demonstrated the expression of LINC00339 in endometriotic lesions for the first time; we found LINC00339 expression was restricted to the lesion foci and absent in surrounding non-lesion tissue. Furthermore, manipulation of LINC00339 expression in endometrial stromal cell lines significantly impacted the expression of genes involved in immune defence pathways. These studies identify a novel mechanism for LINC00339 activity in endometrium and endometriosis, paving the way for future work, which is essential for understanding the pathogenesis of endometriosis.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium RNA, Long Noncoding Case-Control Studies Cell Line Endometriosis Endometriosis Endometriosis Endometrium Endometrium Female Gene Expression Regulation Humans In Situ Hybridization Real-Time Polymerase Chain Reaction RNA, Long Noncoding RNA, Long Noncoding RNA-Seq Signal Transduction

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (56)

Cited by (6)

Source provenance

europepmc
last seen: 2026-07-28T06:14:09.330459+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:24:55.077982+00:00
License: CC0 · commercial use OK