TREM2 Deficiency Attenuates Endometriosis Progression by Inhibiting M2 Polarization of SpMs and Suppressing SIRPα to Enhance Phagocytosis

Qinglin Mo, Mo Q, Lin Y, Yichun Lin, Hao L, Liwei Hao, Zhang L, Leyi Zhang, Zhou Z, Zilu Zhou, Chang R, Ruoshui Chang, Wei Huang, Huang W, Lei Huang, Huang L, Yunxiao Zhou, Zhou Y, Yang Y, Yuxiao Yang, Liu Z, Zhuoran Liu, Wei Zhu, Zhu W, Xue X, Xiaolei Xue, Wu S, Sha Wu
Biology of reproduction · 2026 · doi:10.1093/biolre/ioag159 · PMID:42555090 · W7172537185
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TREM2 deficiency attenuates endometriosis progression by inhibiting M2 polarization of peritoneal macrophages and suppressing SIRPα to enhance phagocytosis.

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Abstract

Endometriosis (EMS) is characterised by a disrupted peritoneal immune microenvironment where M2 macrophage polarization and impaired phagocytosis promote lesion survival. Single-cell RNA-seq (ScRNAseq) of the peritoneal macrophages from EMS patient revealed elevated Triggering Receptor Expressed on Myeloid cells 2 (TREM2) and signal regulatory protein α (SIRPα). The expression of TREM2 and SIRPa is positively correlated, and each are positively correlated with estrogen response. Mouse EMS model was established by intraperitoneal injection of estrogen primed mouse endometrial fragments into recipient female mice. In vitro estrogen treatment of RAW 264.7 cells indicated an increasing TREM2/SIRPα expression and enhancing phagocytosis of lesion cells derived from WT EMS ectopic lesions. Peritoneal macrophage from mice with EMS were analyzed using flow cytometry for the expression of TREM2 and SIRPα. The assessment of F4/80, CD206, and SIRPα expressing cells within the lesions were performed through the implementation of multi-color immunohistochemistry (mIHC). Small peritoneal macrophages (SpMs, F4/80low CD11blow) were markedly increased in the EMS model. Trem2 knockout mice, as the recipient, showed smaller ectopic lesion size and less lesion formation. TREM2 deficiency inhibited SpM polarization into M2 and downregulated their SIRPα expression, while enhancing phagocytic activity. Mechanistically, estrogen pretreatment upregulated TREM2, which correlated with SIRPα upregulation and impaired phagocytosis. Under estrogen exposure, TREM2 knockdown RAW 264.7 cells potentiated phagocytosis. Thus, TREM2 is an important contributing pathogenic factor in promoting EMS by enhancing M2 polarization and suppressing phagocytosis via SIRPα upregulation.

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