Peritoneal Macrophages Modulate Human Granulosa-Luteal Cell Progesterone Production*

J Halme, Jouko Halme, M G Hammond, Mary Hammond, C H Syrop, Craig H. Syrop, Luther M. Talbert, L M Talbert
The Journal of clinical endocrinology and metabolism · 1985 · vol. 61(5) , pp. 912–916 · doi:10.1210/jcem-61-5-912 · PMID:4044779 · W2141265451
article OA: closed CC0 ⤵ 9 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Peritoneal macrophages and blood monocytes increased progesterone production by human granulosa cells, suggesting a luteotropic effect of macrophages on cumulus cells and monocytes on luteal cells.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Macrophages have been identified in the developing corpus luteum in several species, including man, and also constitute approximately 90% of cells in the peritoneal cavity. We studied the effect of peritoneal macrophages or blood monocytes on progesterone (P) synthesis by human granulosa cells from preovulatory follicles obtained at laparoscopy of 14 women undergoing in vitro fertilization. Pooled granulosa cells from follicles with mature ova were isolated by Ficoll-Hypaque gradient centrifugation. Washed granulosa cells (0.75 X 10(5)/ml) were incubated in Dulbecco's Minimum Essential Medium containing 20% calf serum with varying concentrations of pelvic macrophages (0.8-29 X 10(5)/ml) or fresh and mature blood monocytes (0.25-2.5 X 10(5)/ml). P production was determined by RIA of medium at 24-h intervals for 24-48 h. In situ concentrations of pelvic macrophages from 8 patients with tubal infertility increased cumulative P production to 140 +/- 17.8% (mean +/- SEM) of the control values. A similar increase (182 +/- 62.7%) was found with macrophages from 6 patients with endometriosis or unexplained infertility. Both fresh and mature monocytes stimulated P production to 225% and 261% of control values, respectively. Indomethacin (10(-4) M) or monoclonal antibody to somatomedin-C did not prevent stimulation of P production. These results suggest that peritoneal macrophages may exert luteotropic effects on cumulus cells while the ovulated oocyte resides in the tube, and incoming monocytes may be important in stimulating luteal cells in the developing corpus luteum.

My notes (saved in your browser only)

Condition tags

endometriosisinfertility

MeSH descriptors

Corpus Luteum Granulosa Cells Luteal Cells Macrophages Progesterone Adult Cells, Cultured Corpus Luteum Female Fertilization in Vitro Granulosa Cells Humans Indomethacin Indomethacin Insulin-Like Growth Factor I Insulin-Like Growth Factor I Luteal Cells Macrophages Monocytes Monocytes

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (18)

Cited by (9)

Source provenance

europepmc
last seen: 2026-06-11T06:19:48.454388+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:09:40.384591+00:00
License: CC0 · commercial use OK