Luminescent mouse model of endometriosis: three-dimensional morphology of lesions and cytokine profiles

Nanda Yuli Rahmawati, Trish Dinh, Tra Thi Huong Dinh, T. Oikawa, Tomona Oikawa, Akiko Shinogi, Kyoko Ikeda, Masayo Kadota, Masaru Tamura, Takanori Amano, Atsushi Yoshiki
Experimental animals · 2025 · vol. 74(4) , pp. 419–428 · doi:10.1538/expanim.25-0044 · PMID:40350305 · W4410198673
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A luminescent mouse model of endometriosis was developed to non-invasively track lesion growth and characterize lesion morphology and distinct temporal changes in inflammation-related serum cytokines.

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Abstract

The pathophysiology of endometriosis remains incompletely understood, necessitating the development of effective animal models for research. We generated and characterized a luminescent endometriosis mouse model utilizing luminescent B6-CAG-ELuc transgenic mice as uterine tissue donors and B6.Cg-c/c-hr/hr mice as recipients, enabling non-invasive in vivo imaging. Following transplantation of minced uterine tissue fragments into the peritoneal cavity of recipients, we monitored lesion growth via in vivo imaging system on 0, 14, 28, 42 days post transplantation. Morphology of the lesion was observed by dissecting microscopy, X-ray micro-computed tomography, and conventional histology. Inflammation-related serum cytokines were quantified using multiplex immunobeads assay. The growth of endometriotic lesions was efficiently observed by bioluminescence from day 0 through 42 days post transplantation. Comprehensive morphological observations revealed typical endometriotic lesions consisted of multiple fluid-filled cysts lined with single-layered epithelium, associated with glandular epithelial tissues and interstitial stroma. The level of IL-1β, IL-2, IL-6, IL-10, IL-12p70, IFN-γ, and TNF-α was quantified simultaneously in each serum sample to evaluate the temporal changes of each cytokine, showing four distinct patterns: IFN-γ and TNF-α showed continuous increase, IL-12p70 and IL-1β demonstrated gradual increase followed by marked elevation, IL-6 and IL-2 exhibited dramatic increase in later stages, while IL-10 showed transient increase followed by gradual decrease. In conclusion, this luminescent endometriosis mouse model using B6 luminescent transgenic mice as uterine tissue donor and B6.Cg-c/c-hr/hr recipient could be used to investigate comprehensive cytokine profiling in the development of endometriosis.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines Cytokines

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (38)

SciLite annotations

organisms 59
noordeloos 2009062 humans rodents mus sp. rattus sp. human primates transgenic mice transgenic mice mus sp. transgenic mice transgenic mice rodents rodents mus sp. rodents mus sp. mus sp. mus sp. mus sp. mus sp. rodents mus sp. transgenic mice mus sp. mus sp. transgenic mice zea saccharata mus sp. transgenic mice mus sp. transgenic mice mus sp. mus sp. mus sp. mus sp. mus sp. transgenic mice transgenic mice transgenic mice mus sp. mus sp. transgenic mice human transgenic mice transgenic mice transgenic mice human mus sp. rodents mus sp. mus sp. transgenic mice rodents transgenic mice mus sp. mus sp. transgenic mice mus sp.
chemicals 14
water isoflurane wax carbon medetomidine midazolam butorphanol estradiol butorphanol formaldehyde ethanol acid haematoxylin phosphorous acid

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