In vitro effect of danazol on steroidogenesis in developing human follicles. An interim report
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⤵ 8 in-corpus citations
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Danazol inhibited steroid and cAMP production in human granulosa and thecal cells in short-term incubations and suppressed FSH/LH-stimulated progesterone in longer granulosa cell cultures.
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Abstract
In order to show whether or not danazol exerts direct effects on the cells of the human ovarian follicle, follicular granulosa and thecal cells of follicles in different stages of development are currently being investigated in short-term incubation experiments and during more prolonged cell culture. Cells are isolated by free-hand dissection from follicles of 8 to 25 mm diameter, corresponding approximately to cycle day 7 to 14. Granulosa and thecal cells have so far been incubated for 2 to 4 hours in the presence or absence of hCG and/or danazol. A small number of cell cultures of granulosa cells have been performed for up to 6 days in the presence or absence of danazol, hFSH, and hLH, in various combinations. The progesterone (P), estradiol-17 beta (E) and androstenedione (A) content of the incubation or culture medium and the tissue content of cyclic AMP (cAMP) were measured. Danazol (10 micrograms/ml) inhibited thecal cAMP production in most short-term experiments but only the hCG-stimulated cAMP production of granulosa cells. Short-term production of P and E by granulosa and of A and E by thecal cells was also inhibited by danazol in certain circumstances. In tissue cultures of granulosa cells lasting up to 4 days, danazol (1 and 10 micrograms/ml) inhibited both the FSH and LH-stimulated P production. Provided that any unspecific effects of the concentrations of danazol used can be ruled out, the results seem to indicate that this substance may act directly upon steroidogenesis at the ovarian follicular level.
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References (5)
- Endocrine effects of danazol in menstruating women. via openalex
- Evidence that danazol inhibits gonadotropin-induced ovarian steroidogenesis at a point distal to gonadotropin-receptor interaction and adenosine 3',5' cyclic monophosphate formation via openalex
- doi:10.1210/jcem-54-2-436 via openalex
- doi:10.1016/0002-9378(81)90611-6 via openalex
- doi:10.3109/00016348309155789 via openalex
Cited by (8)
- The effect of danazol on pulsatile gonadotropin secretion in women with endometriosis 1991
- The effect of danazol and the LHRH agonist analogue goserelin (Zoladex) on the biological activity of luteinizing hormone in women with endometriosis 1990
- [Suppressive effect of the blast cells of AKR strain mice on antibody formation in vivo and lymphocyte proliferation in vitro] 1988
- Danazol in menorrhagia: a double blind placebo controlled trial 1987
- Inhibitory effects of danazol on steroidogenesis in cultured human granulosa cells 1986
- Direct biphasic effects of danazol on gonadotropin-dependent differentiation of cultured rat granulosa cells 1986
- Danazol treatment of severely symptomatic fibrocystic breast disease and long-term follow-up--the Hjørring project 1984
- Changes in the SHBG concentration during danazol treatment 1984
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- openalex
- last seen: 2026-05-10T10:34:34.680553+00:00
- pubmed
- last seen: 2026-10-08T21:58:02.984372+00:00
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