Hydroxysafflor Yellow A ameliorates adenomyosis by improving IDH1-autophagy-EMT

Xinran Li, zhou Xu, Xu Z, Wang L, Liwei Wang, Xintong Li, Li X, Xuefeng Wu, Wu X, Xudong Wu, Hao Hong, Hong H, Shen Y, Yan Shen, Ronghui Du, Du R
In: Authorea, Inc. · 2023 · doi:10.22541/au.168966930.03401710/v1 · W4384697963
Lab / animal preprint OA: gold CC0
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Hydroxysafflor yellow A ameliorates adenomyosis by promoting autophagy and inhibiting epithelial-mesenchymal transition through targeting isocitrate dehydrogenase 1 and the PI3K-AKT-mTOR pathway.

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Abstract

Background and Purpose: Adenomyosis causes pain, abnormal bleeding, and infertility. Epithelial-mesenchymal transition (EMT) plays a critical role in the pathogenesis of adenomyosis. Current therapies are mainly hormone-dependent and their termination led to aggravations. To develop novel pharmacological treatment is necessary. Safflower was widely used to treat gynecological disease in China, the effect of hydroxysafflower yellow A (HSYA) that is the major active component of Safflower on adenomyosis remains unclear. We investigated whether HSYA ameliorates adenomyosis, and further revealed its potential target and mechanisms. Experimental Approach: Adenomyosis was induced in female ICR mice by oral administration of tamoxifen on days 2-5 after birth. At the 16th week, HSYA were administrated for 3 weeks. Adenomyosis development, autophagy activity, and involved mechanisms were analyzed using HE and Masson staining, western blotting, immunofluorescence, and RNA-sequencing. Furthermore, network pharmacology and molecular docking were applied to assess the potential target of HSYA. Key Results: HSYA ameliorates adenomyosis via promoting autophagy and ameliorating the activation of PI3K-AKT-mTOR. Isocitrate dehydrogenase1(IDH1) was found to be the potential target of HSYA. After IDH1 was knocked down by si-RNA, autophagy was activated, and EMT was attenuated via inhibiting PI3K-AKT-mTOR by HSYA. Conclusions and Implications: HSYA significantly ameliorates adenomyosis, and IDH1-autophagy-EMT plays an important role in the progress of adenomyosis. It will shed new light on a potential novel therapeutic strategy for adenomyosis.

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Condition tags

adenomyosisinfertility

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (33)

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