Redox Status in External Genital Endometriosis

M. V. Osikov, I. V. Kurnosenko, V. A. Kryukov
In: Ural Medical Journal · 2026 · vol. 25(2) , pp. 46–56 · doi:10.52420/umj.25.2.46 · W7159782713
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This study found decreased tissue antioxidant capacity and elevated serum lipid peroxidation products in endometriosis patients, suggesting distinct local and systemic redox profiles for potential diagnosis.

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The paper evaluated redox status in women with external genital endometriosis by measuring lipid peroxidation products (diene conjugates, ketodienes and conjugated trienes, and Schiff bases) and overall antioxidant capacity in serum and in homogenates of ectopic and eutopic endometrial tissue. In a sample of 58 women (10 controls, 12 with mild and 36 with severe endometriosis), spectrophotometric assays showed that in ectopic/eutopic endometrial homogenates, lipid peroxidation markers (DC, KDiST, and SB) were lower and overall antioxidant capacity was decreased compared with controls, while serum showed increased DC and a tendency toward higher antioxidant capacity, indicating systemic oxidative stress. As a stage-related finding, DC in the heptane phase and KDiST in the isopropanol phase were lower in mild endometriosis than in severe disease, with statistical analyses including Kruskal–Wallis and post hoc testing. The authors conclude that differences in redox status may support diagnosis and note that lower lipid peroxidation products in ectopic tissue compared with eutopic tissue may relate to absence of peritoneal microbiota and adaptive mechanisms in lesions. This paper is centrally about endometriosis — specifically the redox status (oxidative stress and antioxidant capacity) in external genital endometriosis with potential diagnostic marker value.

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Abstract

Introduction . Oxidative stress is a key factor in external endometriosis. The search for peripheral blood markers is promising due to the lack of non-invasive diagnostics. Existing data are contradictory and require correlation with local changes in lesions. Aim is to assess the levels of lipid peroxidation (LPO) products and total antioxidant capacity (TAC) in serum, ectopic and eutopic endometrium in patients with endometriosis and to evaluate their potential as diagnostic markers. Materials and methods . The study included 58 women (control — 10 patients; mild and severe endometriosis — 12 and 36 patients, respectively). Spectrophotometric analysis of tissue homogenates and serum measured lipid peroxidation products: diene conjugates (DC), ketodienes and conjugated trienes (KD&CT), Schiff bases (SB), and TAC. Statistical analysis used Shapiro-Wilk, Levene, Fisher, Kruskal-Wallis, and post hoc Dunnett-Steel-Critchlow-Fligner tests. Results . In endometrial homogenates, levels of DC, KD&CT and SB were significantly lower than in control. Conversely, serum DC and TAC levels were statistically higher in endometriosis patients. Tissue TAC was decreased, while serum TAC showed a tendency to increase. In homogenates, DC (heptane phase) and KD&CT (isopropanol phase) levels were lower in mild versus severe disease. Conclusion . In endometriosis, local oxidative stress in tissues is characterized by decreased TAC, indicating antioxidant depletion, while systemic circulation shows elevated DC. The reduced lipid peroxidation in ectopic versus eutopic endometrium may result from the absence of abdominal microbiota and local adaptive mechanisms. These differences in redox status may be used for the diagnosis of the disease.
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Особенности редокс-статуса при наружном генитальном эндометриозе https://doi.org/10.52420/umj.25.2.46 EDN: OASMJY Аннотация Введение. Один из ведущих факторов развития эндометриоза — окислительный стресс (ОС), показателями которого являются продукты перекисного окисления липидов (ПОЛ) и общая антиоксидантная способность (ОАС). В связи с отсутствием малоинвазивных методов диагностики эндометриоза в качестве сывороточных маркеров заболевания перспективен поиск показателей активности ОС. Цель исследования — оценить содержание продуктов ПОЛ и ОАС в сыворотке, эктопическом и эутопическом эндометрии у пациенток с эндометриозом и возможность их использования в качестве диагностических маркеров. Материалы и методы. В исследование включено 58 женщин (контрольная группа — 10 человек; легкая и тяжелые формы эндометриоза — 12 и 36 пациенток соответственно). В гомогенатах эндометрия и сыворотке при спектрофотометрии определялись продукты ПОЛ (диеновые конъюгаты (ДК), кетодиены и сопряженные триены (КДиСТ), основания Шиффа (ОШ)), ОАС. Для статистического анализа использовались критерии Шапиро — Уилка, Левена, Фишера, Краскела — Уоллиса и post hoc критерий Даннета — Стила — Кричлоу — Флигнера. Результаты. В гомогенатах эндометрия у пациенток с эндометриозом уровни ДК, КДиСТ и ОШ были статистически значимо ниже, чем у пациенток из контрольной группы. При этом в сыворотке у пациенток с эндометриозом уровни ДК были статистически значимо выше. ОАС в гомогенатах была снижена при эндометриозе, тогда как в сыворотке наблюдалась тенденция к ее повышению. В гомогенатах уровни ДК гептановой фазы и КДиСТ изопропанольной фазы при легком эндометриозе были ниже, чем при тяжелом течении. Заключение. Признаками ОС при эндометриозе в гомогенатах является снижение уровня ОАС, связанное с расходованием компонентов антиоксидантной системы; в сыворотке — повышение уровня ДК и ОАС, что свидетельствует о системном ОС. Более низкий уровень продуктов ПОЛ в гомогенатах эктопического эндометрия, в сравнении с эутопическим, может быть обусловлен отсутствием микробиоты в брюшной полости и приспособительными механизмами в очагах эндометриоза. Различия в редокс-статусе могут использоваться для диагностики заболевания. Об авторах М. В. ОсиковРоссия Михаил Владимирович Осиков — доктор медицинских наук, профессор, заведующий кафедрой патофизиологии, Южно‑Уральский государственный медицинский университет; руководитель отдела научной работы, Челябинская областная клиническая больница Челябинск Конфликт интересов: М. В. Осиков — член редакционной коллегии «Уральского медицинского журнала»; не принимал участия в рассмотрении и рецензировании материала, а также принятии решения о его публикации И. В. Курносенко Россия Илона Владимировна Курносенко — доктор медицинских наук, доцент, заведующий кафедрой акушерства и гинекологии, Южно‑Уральский государственный медицинский университет; акушер‑гинеколог отделения патологии беременных, Областной перинатальный центр Челябинск Конфликт интересов: Остальные авторы заявляют об отсутствии явных и потенциальных конфликтов интересов В. А. Крюков Россия Владимир Александрович Крюков — ассистент кафедры патофизиологии, Южно‑Уральский государственный медицинский университет; акушер‑гинеколог гинекологического отделения, Челябинская областная клиническая больница Челябинск Конфликт интересов: Остальные авторы заявляют об отсутствии явных и потенциальных конфликтов интересов Список литературы 1. Özbek DÜ, Karakuş S, Bakır S. Levels of oxidative stress and apoptosis-related biomarkers in endometriosis. Cukurova Medical Journal. 2023;48(2):480–488. DOI: https://doi.org/10.17826/cumj.1247227. 2. 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(In Russ.) https://doi.org/10.52420/umj.25.2.46. EDN: OASMJY JATS XML

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