Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines

Miroslava Rabajdová, Ivana Špaková, Zuzana Klepcová, Lukáš Smolko, Michaela Abrahamovská, Peter Urdzík, Mária Mareková
Scientific reports · 2021 · vol. 11(1) , pp. 19086 · doi:10.1038/s41598-021-98512-9 · PMID:34580366 · W3204561709
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

Zinc(II) niflumato complex reduced proliferation and MMP activity in immortalized human endometriotic cells compared to control endometrial cells.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-10 ⓘ

This study tested the cytotoxic and molecular effects of a zinc(II) niflumato complex with neocuproine (Zn-Nif) on an immortalized human endometriotic cell line (12Z) compared with a control immortalized human endometrial stromal cell line (hTERT), using xCELLigence-based monitoring over ~72 hours and viability assessment (Trypan Blue). Treatment slowed proliferation in 12Z cells, while hTERT cells did not show similar behavior, and the authors report that Zn-Nif likely reduces pro-inflammatory pathway effects (attributed to NSAID activity) alongside zinc-mediated modulation of ROS and ER2 regulation and changes in MMP activity. A stated caveat is that the work is performed in immortalized in vitro cell lines rather than in endometriotic tissue or patients. This paper is centrally about endometriosis—specifically Zn-Nif effects on MMP activity and gene expression in human endometriotic and control endometrial cell lines.

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Abstract

Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn( neo )( nif ) 2 ] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on control immortalized human endometrial stromal cell line (hTERT) was performed using xCELLigence technology for approximately 72 h following the treatment with Zn-Nif as well as cell viability Trypan Blue Assay. 12Z cell line proliferated more slowly compared to unaffected cells, whereas hTERT cells did not show similar behavior after treatment. The complex probably reduces the effect of pro-inflammatory pathways due to the effect of NSAID, while presence of zinc might reduce the level of ROS and regulate ER2 levels and MMP activity. The observed effects and high selectivity for rapidly proliferating cells with increased inflammatory activity suggest a good prognosis of successful decrease of endometriosis stage with this complex.

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Condition tags

mesh:D004715endometriosisinfertility

MeSH descriptors

Endometriosis Matrix Metalloproteinases Organometallic Compounds Phenanthrolines Zinc Cell Line Cell Survival Cell Survival Drug Evaluation, Preclinical Endometriosis Endometriosis Endometrium Endometrium Endometrium Female Gene Expression Profiling Gene Expression Regulation Gene Expression Regulation Humans Matrix Metalloproteinases

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