Chronic pelvic pain associated with autoimmunity and systemic and peritoneal inflammation and treatment with immune modification.

John C Thomson, David B. Redwine, М. Л. Богомазова, Irina Zhuravleva, А. М. Исмаилов, Е. Г. Комелькова, Л. Н. Лесинкер, О. В. Отческая, С. В. Петров, Ю. В. Соломатин, А. М. Теплышева, Т. Н. Вдовиченко, Е. Л. Хромых, A S Chukanova, А. В. Лебедева, С. Г. Бурд, А. Н. Бойко
In: Эпилепсия и пароксизмальные состояния · 2005 · vol. 3(10) , pp. 745–58 · doi:10.1016/0378-4347(91)80403-y · W1874886
OA: closed CC0 ⤵ 14 in-corpus citations
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This paper describes a novel affinity chromatography method using Polybrene and a volatile buffer system for isolating and purifying glycosaminoglycans from complex biological samples like plasma.

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Abstract

A new system for the isolation and purification of glycosaminoglycans (GAGs) and related molecules from complex systems such as plasma is described. Affinity chromatography which exploits the very high affinity between the polymeric base Polybrene and sulphated polysaccharides was used. A novel volatile buffer system composed of ammonium formate and formic acid, which allows the complete recovery of samples, was developed, and elution conditions were optimised for the separation and purification of GAGs of different charge densities. Using this system the losses associated with dialysis and desalting, frequently necessary preliminaries to further analysis, are avoided.

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chronic_pelvic_pain

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