LncRNA MIR22HG is downregulated in adenomyosis and upregulates miR‐2861 through demethylation to inhibit endometrial cell proliferation

Ke Yu, Yu K, Cui S, Shuna Cui, Tongmin Xue, Xue T
Journal of Obstetrics and Gynaecology Research · 2021 · vol. 47(5) , pp. 1837–1845 · doi:10.1111/jog.14665 · PMID:33624428 · W3129607437
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MIR22HG is downregulated in adenomyosis, upregulating miR-2861 via demethylation to inhibit endometrial cell proliferation by downregulating STAT3 and MMP2.

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Abstract

AIM: Endometrial cell proliferation plays a critical role in adenomyosis. It has been reported that MIR22HG and miR-2861 play similar roles in regulating endometrial cell proliferation, indicating their involvement in adenomyosis. This study aimed to investigate the potential involvement of MIR22HG and miR-2861 in adenomyosis. METHODS: Endometrial biopsy was collected from both adenomyosis (n = 45) and the healthy controls (n = 45). The expression of MIR22HG and miR-2861 in biopsies were determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The relationship between MIR22HG and miR-2861 in endometrial cells was analyzed by RT-qPCR. Methylation-specific PCR (MSP) was performed to analyze the effects of overexpression of MIR22HG on the expression of miR-2861. Western-blot assays were performed to illustrate the effect of MIR22HG, miR-2861, STAT3, and MMP2 on adenomyosis. Luciferase report assay was performed to analyze the interactions among miR-2861, STAT3, and MMP2. The role of MIR22HG and miR-2861 in regulating the proliferation of endometrial cells was analyzed by cell proliferation assay. RESULTS: The expression of MIR22HG and miR-2861 in adenomyosis did not change with menstrual cycle. MIR22HG and miR-2861 were significantly downregulated in adenomyosis and they were significantly and positively correlated with each other. In endometrial cells, overexpression of MIR22HG upregulated the expression of miR-2861 and decreased methylation of miR-2861 gene. MIR22HG and miR-2861 downregulated STAT3 and MMP2 to inhibit the proliferation of endometrial cells. In addition, overexpression of both MIR22HG and miR-2861 showed stronger effects. CONCLUSION: MIR22HG is downregulated in adenomyosis and upregulates miR-2861 through demethylation to inhibit endometrial cell proliferation.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis MicroRNAs MicroRNAs MicroRNAs RNA, Long Noncoding RNA, Long Noncoding Cell Proliferation Demethylation Female Gene Expression Regulation, Neoplastic Humans

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