Regulatory mechanism of GPER in the invasion and migration of ectopic endometrial stromal cells in endometriosis

Hongyan Shi, Kejun Xu, Mengna Huang, Meiya Mao, Jilan Ou
Women & health · 2023 · vol. 64(2) , pp. 109–120 · doi:10.1080/03630242.2023.2296522 · PMID:38148599 · W4390270378
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GPER promotes the invasion and migration of ectopic endometrial stromal cells in endometriosis, and its expression is negatively regulated by miR-16-5p and miR-103a-3p.

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Abstract

Endometriosis (EMS) is a chronic inflammatory disorder of high incidence that causes serious reproductive consequences. High estrogen production is a consistently observed endocrine feature of EMS. The present study aims to probe the molecular mechanism of G protein-coupled estrogen receptor 1 (GPER) in the invasion and migration of ectopic endometrial stromal cells (Ect-ESCs) and provides a new rationale for EMS treatment. Eutopic and ectopic endometrial tissues were collected from 41 EMS patients, and primary ESCs were separated. GPER, miR-16-5p, and miR-103a-3p levels in cells and tissues were determined by qRT-PCR or Western blot assay. Cell viability, proliferation, invasion, and migration were evaluated by CCK-8, colony formation, and Transwell assays. The upstream miRNAs of GPER were predicted by databases, and dual-luciferase assay was performed to validate the binding of miR-16-5p and miR-103a-3p to GPER 3'UTR. GPER was highly expressed in EMS tissues and Ect-ESCs. Inhibition of GPER mitigated the proliferation, invasion, and migration of Ect-ESCs. GPER was regulated by miR-16-5p and miR-103a-3p. Overexpression of miR-16-5p and miR-103a-3p negatively regulated GPER expression and inhibited the invasion and migration of Ect-ESC. In conclusion, GPER promoted the invasion and migration of Ect-ESCs, which can be reversed by upstream miR-16-5p and miR-103a-3p.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis MicroRNAs MicroRNAs MicroRNAs MicroRNAs MicroRNAs MicroRNAs MicroRNAs Cell Movement Cell Movement Cell Movement Cell Movement Cell Movement Cell Movement Estrogens Estrogens

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