Cytotoxic activity of peripheral blood mononuclear cells in patients with endometriosis: A cross-sectional study

Raden Muharam, Arleni Bustami, Bustami A, Indra Gusti Mansur, Gusti Mansur I, Zulkifli Jacoeb T, Teuku Z Jacoeb, Teuku Zulkifli Jacoeb, Jérôme Giustiniani, Giustiniani J, Jerome Giustiniani, Schiavon V, Valérie Schiavon, Valerie Schiavon, Armand Bensussan
International journal of reproductive biomedicine · 2022 · vol. 20(8) , pp. 691–700 · doi:10.18502/ijrm.v20i8.11758 · PMID:36313261 · PMC9596928 · W4295095351
article OA: gold CC0 ⤵ 4 in-corpus citations
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-07-09 ⓘ

This study investigated T lymphocyte and NK cell activity in women with endometriosis, finding lower co-stimulatory molecule expression and reduced IL-2 response compared to controls.

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⚙ AI-generated deep summary by claude@2026-07, 2026-07-09 · read from full text ⓘ

This cross-sectional study compared peripheral blood mononuclear cell (PBMC) immune phenotypes and cytotoxic activity between 7 women with laparoscopically and histopathologically confirmed endometriosis and 7 laparoscopic controls without endometriosis, using flow cytometry for CD28 and CD160 and IL-2 stimulation followed by cytotoxicity testing against Daudi and K562 targets. The main finding was that CD3+CD28+ T cells were significantly lower in the endometriosis group before stimulation, while other lymphocyte subpopulations did not differ at baseline, and IL-2 did not change NK cell subpopulations in either group after 4 days. IL-2 enhanced cytotoxicity of PBMC effector cells against both target cell lines in women with endometriosis across tested doses, with significant increases observed for T and NK cell cytotoxicity. A key limitation explicitly reflected by the study design is the small sample size (n=7 per group). This paper is centrally about endometriosis — it examines altered CD28/CD160-related T-cell/NK-cell cytotoxic activity in IL-2-stimulated PBMCs from women with endometriosis.

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Abstract

BACKGROUND: Endometriosis is believed to be associated with dysfunction of the lymphocyte population and cytotoxicity of natural killer (NK) cells, induced by the production of interleukin-2 (IL-2). OBJECTIVE: This study aimed to investigate T lymphocytes and NK cell activity in the peripheral blood mononuclear cells (PBMCs) of women with endometriosis. MATERIALS AND METHODS: PBMCs were obtained from the peripheral venous blood samples of 14 women with and without endometriosis (n = 7 for each group). Then, the PBMCs were co-cultured for 4 days and were treated with recombinant IL-2 for cytotoxic activity toward target cells (Daudi and K562 cells). The cytotoxicity activity was determined using the 51 chromium release assay before and after stimulation. Flow cytometry measurement was used to examine the expression of T lymphocytes and NK cells before and after being treated with IL-2. RESULTS: The concentration of CD3+CD28+ (co-stimulatory) was significantly lower in the endometriosis group (65.62 ± 5.38) compared to in its counterpart (50.24 ± 4.22) (p = 0.04) before stimulation. However, no significant differences were observed in any other T lymphocytes and NK cells. It was also found that there was a significant increase of CD3-CD28+ after treatment with IL-2 only in the healthy control but not in women with endometriosis. CONCLUSION: Increased expression of CD160 and decreased CD28 play a role in inhibiting NK cell activation and T cell response in women with endometriosis.
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In conclusion, this study, combined with a previous one (16), demonstrated that the increased expression of CD160 and decreased expression of CD28 may play a role in inhibiting NK cell activation and T cell response in endometriosis.

Coi Statement

The authors declare that there is no conflict of interest.

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endometriosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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