Comparison of the Effects of Dienogest and Leuprolide Acetat on Serum Interferon (IFN-Ɣ) Levels in a Mouse Model of Endometriosis (Mus musculus)

Maria Mali, Siskanita Nur Fitriana, Sutrisno Sutrisno, Husnul Khotimah, I Wayan Arsana Wıyasa, I Wayan Agung Indrawan, Maharani Maharani
In: Asian Journal of Health Research · 2023 · vol. 2(2) , pp. 3–9 · doi:10.55561/ajhr.v2i2.111 · W4385782920
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

Leuprolide acetate significantly reduced serum IFN-Ɣ levels in a mouse model of endometriosis, while dienogest treatment increased these levels.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-10 · read from full text ⓘ

The paper compared the effects of dienogest versus leuprolide acetate on serum interferon-gamma (IFN-γ) levels in a mouse model of endometriosis, using a post-test only control group design with four groups and measuring IFN-γ by ELISA after 14 days of treatment. Leuprolide acetate significantly decreased serum IFN-γ, whereas dienogest increased serum IFN-γ levels. A key caveat explicitly implied by the design is that it used a post-test only approach, limiting assessment of baseline changes over time, and the study reports only serum IFN-γ outcomes analyzed by one-way ANOVA. This paper is centrally about endometriosis — it directly tests dienogest and leuprolide effects on IFN-γ in an endometriosis mouse model.

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Abstract

Introduction: Endometriosis is a medical condition in which there is abnormal growth of tissue resembling the endometrium outside the uterus. It can cause symptoms of pain and/or infertility. The current medical therapy focuses on reducing estradiol levels or stimulating progesterone response. One of the most effective options for medical treatment is the use of GnRH analogs. Dienogest have a direct anti-inflammatory effect on endometriotic stromal cells. However, a comparison of the anti-inflammatory effects of these drugs on IFN-Ɣ has not been previously investigated. Therefore, this study aims to compare the effects of reducing IFN-Ɣ by both Dienogest and Leuprolide Acetat in a mouse model. Material and Methods: This study employed a post-test only control group design and involved 4 groups, namely the negative control group, positive control group, and two treatment groups: one group administered Dienogest at a dosage of 0.0052 milligrams per day for 14 days, and the other administered Leuprolide Acetat at a dosage of 0.00975 milligrams once every 5 days for a period of 14 days. Serum IFN-Ɣ levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA). The data were subsequently analyzed using IBM SPSS 25 with One-Way ANOVA test. Results: This study demonstrated that Leuprolide Acetat significantly decreased serum levels of IFN-Ɣ, whereas Dienogest actually increased the levels . Conclusion: In a mouse model of endometriosis (Mus musculus), Leuprolide Acetat effectively reduced the levels of IFN-Ɣ (pro-inflammatory). Conversely, Dienogest increased the levels of IFN-Ɣ (anti-inflammatory) in the mouse model of endometriosis.
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Keywords

Endometriosis, IFN-Ɣ, Dienogest, Leuprolide AcetatAbstract

Introduction

Endometriosis is a medical condition in which there is abnormal growth of tissue resembling the endometrium outside the uterus. It can cause symptoms of pain and/or infertility. The current medical therapy focuses on reducing estradiol levels or stimulating progesterone response. One of the most effective options for medical treatment is the use of GnRH analogs. Dienogest have a direct anti-inflammatory effect on endometriotic stromal cells. However, a comparison of the anti-inflammatory effects of these drugs on IFN-Ɣ has not been previously investigated. Therefore, this study aims to compare the effects of reducing IFN-Ɣ by both Dienogest and Leuprolide Acetat in a mouse model.

Material and methods

This study employed a post-test only control group design and involved 4 groups, namely the negative control group, positive control group, and two treatment groups: one group administered Dienogest at a dosage of 0.0052 milligrams per day for 14 days, and the other administered Leuprolide Acetat at a dosage of 0.00975 milligrams once every 5 days for a period of 14 days. Serum IFN-Ɣ levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA). The data were subsequently analyzed using IBM SPSS 25 with One-Way ANOVA test.

Results

This study demonstrated that Leuprolide Acetat significantly decreased serum levels of IFN-Ɣ, whereas Dienogest actually increased the levels .

Conclusion

In a mouse model of endometriosis (Mus musculus), Leuprolide Acetat effectively reduced the levels of IFN-Ɣ (pro-inflammatory). Conversely, Dienogest increased the levels of IFN-Ɣ (anti-inflammatory) in the mouse model of endometriosis. Downloads Published Issue Section License Copyright (c) 2023 Maria Antonia Barbara Batu Mali, Siskanita Nur Fitriana, Sutrisno Sutrisno, Husnul Khotimah, I Wayan Arsana Wiyasa, I Wayan Agung Indrawan, Maharani Maharani This work is licensed under a Creative Commons Attribution 4.0 International License.

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Condition tags

endometriosisinfertility

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (27)

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