Peripheral blood subpopulations of Bregs producing IL-35 in women with endometriosis

Anna Sławek, Daria Lorek, A Kedzierska, Paulina Kubik, Pajak J, J Pajak, Jaroslaw Pajak, Chrobak A, Agnieszka Chrobak, Anna Chełmońska‐Soyta, Anna Chelmonska-Soyta
American journal of reproductive immunology (New York, N.Y. : 1989) · 2022 · vol. 89(3) , pp. e13675 · doi:10.1111/aji.13675 · PMID:36579639 · W4313280002
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This study measured Breg subpopulations and IL-35 production in women with endometriosis, finding decreased B10 cells and plasmablasts but increased IL-35 production in these cells compared to healthy controls.

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Abstract

PROBLEM: Interleukin 35 (IL-35) is involved in the pathogenesis of endometriosis by suppressing immunoreaction and promoting endometrial cell proliferation. It may also be an essential cytokine in forming the immunosuppressive functions of regulatory B lymphocytes (Bregs). The involvement of Bregs in the pathogenesis of endometriosis has not been previously investigated. In this study, we determined the frequencies of different Breg subpopulations, namely, B10, immature B-cells, and plasmablasts, and their abilities to produce IL-35 in women with endometriosis compared to healthy women. METHODS: The frequencies of different subpopulations of Bregs producing IL-35 were measured in the peripheral blood of women with endometriosis (total pool), women with deep infiltration endometriosis (DIE), women with ovarian endometriosis, and healthy women as a control by flow cytometry. RESULTS: We observed a decrease in the percentage of B10 cells and plasmablasts in women with endometriosis and an increase in the percentage of these Breg populations producing IL-35 in the same experimental group. Interestingly, we also revealed that women with DIE had increased percentages of B10 cells and plasmablasts producing IL-35. CONCLUSION: Taken together, our findings are the first to reveal the frequencies of different subpopulations of Bregs producing IL-35 in women with endometriosis. The results suggest that IL-35 expression in B lymphocytes could be used as a peripheral marker of endometriosis; however, further studies are needed.

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Condition tags

endometriosisdie_deep_infiltrating

MeSH descriptors

B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory

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References (39)

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