The content of cytokines IL-6, IL-8, TNF-α, IL-4 and the level of expression in macrophages CD86 and CD163 in peritoneal fluid has a reverse correlation with the degree of severity of external genital endometriosis

The content of cytokines IL-6, IL-8, TNF-α, IL-4 and the level of expression in macrophages CD86 and CD163 in peritoneal fluid has a reverse correlation with the degree of severity of external genital endometriosis 1. Academician Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; Koltsov Institute of Developmental Biology, Moscow, Russia 2. Academician Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia Concentrations of eight different cytokines and the level of expression of CD86 and CD163 macrophages were studied in peritoneal fluid in women with endometriosis. It was found that the concentration of both inflammatory (IL-6, IL-8, TNF-α) and anti-inflammatory cytokines (IL-4) as well as the level of macrophage expression of the proinflammatory marker CD86 and anti-inflammatory marker CD163 increased in women with mild external genital endometriosis (1-2 stage), and did not differ from the control group in women with severe endometriosis (3-4 stage). The content of IL-2, IL-10, CM-CSF and IFN-γ in the peritoneal fluid of women with endometriosis did not differ significantly from the control group. The results of the study indicate that the development of external genital endometriosis may be based on insufficient both inflammatory and anti-inflammatory activity of macrophages in the peritoneal fluid. Krasnyi A.M., Sadekova A.A., Sefihanov T.G., Vtorushina V.V., Krechetova E.G., Khilkevich E.G., Arakelyan A.S., Pavlovich S.V. (2019) The content of cytokines IL-6, IL-8, TNF-α, IL-4 and the level of expression in macrophages CD86 and CD163 in peritoneal fluid has a reverse correlation with the degree of severity of external genital endometriosis. Biomeditsinskaya Khimiya, 65(5), 432-436. Krasnyi A.M. et al. The content of cytokines IL-6, IL-8, TNF-α, IL-4 and the level of expression in macrophages CD86 and CD163 in peritoneal fluid has a reverse correlation with the degree of severity of external genital endometriosis // Biomeditsinskaya Khimiya. - 2019. - V. 65. -N 5. - P. 432-436. Krasnyi A.M. et al., "The content of cytokines IL-6, IL-8, TNF-α, IL-4 and the level of expression in macrophages CD86 and CD163 in peritoneal fluid has a reverse correlation with the degree of severity of external genital endometriosis." Biomeditsinskaya Khimiya 65.5 (2019): 432-436. Krasnyi, A. M., Sadekova, A. A., Sefihanov, T. G., Vtorushina, V. V., Krechetova, E. G., Khilkevich, E. G., Arakelyan, A. S., Pavlovich, S. V. (2019). The content of cytokines IL-6, IL-8, TNF-α, IL-4 and the level of expression in macrophages CD86 and CD163 in peritoneal fluid has a reverse correlation with the degree of severity of external genital endometriosis. Biomeditsinskaya Khimiya, 65(5), 432-436. Bacci M., Capobianco A., Monno A., Cottone L., Di Puppo F., Camisa B., Mariani M., Brignole C., Ponzoni M., Ferrari S., Panina-Bordignon P., Manfredi A.A., Rovere-Querini P. (2009) Am. J. Pathol., 175(2), 547-556. CrossRef Scholar google search Itoh F., Komohara Y., Takaishi K., Honda R., Tashiro H., Kyo S., Katabuchi H., Takeya M. (2013) Fertility & Sterility, 99(6), 1705-1713. CrossRef Scholar google search Cheong Y.C., Shelton J.B., Laird S.M., Richmond M., Kudesia G., Li T.C., Ledger W.L. (2002) Hum. Reprod., 17(1), 69-75. CrossRef Scholar google search Pizzo A., Salmeri F.M., Ardita F.V., Sofo V., Tripepi M., Marsico S. (2002) Gynecologic Obstetric Invest., 54(2), 82-87. CrossRef Scholar google search Harada T., Yoshioka H., Yoshida S., Iwabe T., Onohara Y., Tanikawa M., Terakawa N. (1997) Am. J. Obstetr. Gynecol., 176(3), 593-597. CrossRef Scholar google search