Additional file 2 of CCL20/CCR6 axis mediates macrophages to promote proliferation and migration of ESCs by blocking autophagic flux in endometriosis
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This study validated transfection efficiency of si-CCR6, TFEB-OE, and Ad-LC3 using qRT-PCR and fluorescence microscopy.
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Abstract
Additional file 2: Fig. 2. Validation of transfection efficiency. (A) Transfection efficiency of si-CCR6 assessed by qRT-PCR. (B) Transfection efficiency of TFEB-OE assessed by qRT-PCR. (C) Transfection efficiency of Ad-LC3 at different MOI visualized under fluorescence microscopy. (original magnification 200×) Data are presented as the mean ± SD of n=3 independent experiments. Statistical analysis was performed using Mann-Whitney test. ****p<0.0001, **p<0.01.
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