MiR-30c-5p Directly Targets MAPK1 to Regulate the Proliferation, Migration and Invasion of Adenomyotic Epithelial Cells in Adenomyosis

Airong Zhang, Xiujuan Liu, Juan Wang, Kui Deng, Jianghua Wang
Twin research and human genetics : the official journal of the International Society for Twin Studies · 2021 · vol. 24(1) , pp. 22–28 · doi:10.1017/thg.2021.11 · PMID:33775270 · W3150800045
article OA: bronze CC0 ⤵ 2 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

MiR-30c-5p is downregulated in adenomyosis and directly targets MAPK1 to inhibit the proliferation, migration, and invasion of adenomyotic epithelial cells.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study examined miR-30c-5p expression in ectopic and eutopic endometrial tissues from 23 adenomyosis patients undergoing hysterectomy, and evaluated its effects on adenomyotic epithelial cells using gain- and loss-of-function assays. The authors found miR-30c-5p was down-regulated in adenomyosis tissues and adenomyotic epithelial cells, correlating with dysmenorrhea, longer symptom duration, and greater menstrual bleeding, and that miR-30c-5p overexpression inhibited proliferation, migration, and invasion in vitro while knockdown had opposite effects. Using luciferase reporter assays plus qRT-PCR and Western blot, they confirmed MAPK1 as a direct target mediating these changes, but the work relied on a relatively small clinical sample and in vitro functional assays without in vivo validation. This paper is centrally about endometriosis/adenomyosis—specifically adenomyosis, focusing on how miR-30c-5p regulates adenomyotic epithelial proliferation, migration, and invasion via direct targeting of MAPK1.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

The purpose of our study was to elucidate the functions of miR-30c-5p on adenomyosis for exploring novel treatment strategies. We first detected the expression of miR-30c-5p in clinical adenomyotic tissues and isolated endometrial cells from adenomyotic tissues. Next, gain and loss-of-function assays were performed to detect the effect of miR-30c-5p on adenomyotic endometrial cells. Further, luciferase assay and real-time polymerase chain reaction as well as western blot were conducted to investigate the potential target of miR-30c-5p; and transwell assay, wound-healing assay and CCK-8 assay were used to evaluate the effects of miR-30c-5p and its target on regulating biological functions of adenomyotic endometrial cells. Our results found that miR-30c-5p was down-regulated in both adenomyosis tissues and adenomyotic epithelial cells, which correlated with dysmenorrhea, longer duration of symptoms and more menstrual bleeding. Moreover, the overexpression of miR-30c-5p inhibited the proliferation, migration and invasion of adenomyotic epithelial cells, where miR-30c-5p knockdown had an opposite effect. Furthermore, we confirmed mitogen-activated protein kinase 1 (MAPK1) was one of the direct targets of miR-30c-5p, indicating its important role in miR-30c-5p-mediated suppression of proliferation, invasion and migration in adenomyotic epithelial cells. This study showed that the interaction of miR-30c-5p with MAPK1 can regulate the proliferation, invasion and migration in adenomyotic epithelial cells.

My notes (saved in your browser only)

Condition tags

adenomyosisdysmenorrhea

MeSH descriptors

Adenomyosis Adenomyosis MicroRNAs MicroRNAs Cell Movement Cell Movement Cell Proliferation Cell Proliferation Epithelial Cells Female Gene Expression Regulation, Neoplastic Humans Mitogen-Activated Protein Kinase 1

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (39)

Cited by (2)

Source provenance

europepmc
last seen: 2026-06-04T01:30:01.192114+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:24:43.494969+00:00
License: CC0 · commercial use OK