miR-146b level and variants is associated with endometriosis related macrophages phenotype and plays a pivotal role in the endometriotic pain symptom

Zhang Z, Zengzhen Zhang, Huiqing Li, Li H, Zhao Z, Zhao Zhitao, Zhitao Zhao, Baofeng Gao, Gao B, Meng L, Li Meng, Xianguang Feng, Feng X
Taiwanese journal of obstetrics & gynecology · 2019 · vol. 58(3) , pp. 401–408 · doi:10.1016/j.tjog.2018.12.003 · PMID:31122533 · W2945500373
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Increased miR-146b expression and specific variants are associated with endometriosis, macrophage phenotype, and endometriotic pain by negatively regulating inflammation via IRF5.

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Abstract

OBJECTIVE: The aim of this study was to explore the effect of miR-146b expression and variants on endometriosis and its associated pain symptom. MATERIALS AND METHODS: Genotyping and expression of miR-146b was performed on 74 endometriosis patients and 23 healthy controls. ESCs were subsequently co-cultured with peripheral blood (PB)-derived monocytes (PBMC)-driven macrophages. After overexpression and inhibition of miR-146b, cytokine production from the macrophages were determined by enzyme-linked immunosorbent assay (ELISA). Western blot were done to measure the regulation of IRF5 by miR-146b. RESULTS: We found that miR-146b expression was increased in PF supernatant and PF CD14 + monocytes/Macrophages of endometriosis patients, with endometriosis patients with pain (EPWP) showing higher miR-146b expression compared with the endometriosis patients without pain (EPNP). CT/CC genotype of miR-146b rs1536309 was associated with the risk of pain symptom of endometriosis. For the function studies, we found that miR-146b was involved in the negative regulation of inflammation through attenuating IRF5 expression. Macrophages from patients who carries CT/CC genotype of miR-146b rs1536309 showed decreasing miR-146b expression and enhancement of the ability of pro-inflammation. CONCLUSIONS: Our findings suggest an important role of miR-146b level and variants in endometriosis that helps to regulate the process of endometriosis and its associated pain.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis MicroRNAs MicroRNAs Pain Adult Adult Case-Control Studies Case-Control Studies Endometriosis Endometriosis Female Female Humans Humans Interferon Regulatory Factors Interferon Regulatory Factors Macrophages Macrophages Macrophages Pain

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