Fresh mouse peritoneal macrophages have low scavenger receptor activity

J. G. Kim, Kim JG, Channa Keshava, C Keshava, A A Murphy, Murphy AA, R E Pitas, Pitas RE, Robert E. Pitas, Srinivasan Parthasarathy, Parthasarathy S, S Parthasarathy
Journal of lipid research · 1997 · vol. 38(11) , pp. 2207–2215 · doi:10.1016/s0022-2275(20)34934-8 · PMID:9392418 · W2402457099
Lab / animal OA: hybrid CC0 ⤵ 13 in-corpus citations
🔓 Open OA copy View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

Fresh mouse peritoneal macrophages exhibit low scavenger receptor activity that increases with in vitro culture due to posttranscriptional regulation, not changes in mRNA expression.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Peritoneal macrophages are easily isolated by lavage, suggesting that they are either nonadherent or weakly adherent in situ. Cultured macrophages express class A scavenger receptors (SCR), which mediate Ca2+-independent adhesion in vitro. We examined fresh peritoneal macrophages from mice and from women with endometriosis to determine whether the adherence of these cells was associated with increased expression of class A SCR. Fresh human macrophages were not immunoreactive to SCR antibodies; however, SCR immunoreactivity increased with time in culture. Fresh mouse and human macrophages took up minimal amounts of 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI)-acetyl-low density lipoproteins (Ac-LDL), a class A SCR ligand. Murine macrophages in culture for 24-72 h internalized four times more Ac-LDL than fresh cells. Cells cultured for 2 days incorporated 3.2 times more [14C] oleate than freshly isolated cells (55.7 +/- 7.9 versus 17.6 +/- 3.0 nmol/mg cell protein). In contrast to SCR activity, mouse macrophage SCR mRNA expression was similar in freshly isolated macrophages and those cultured for 3 days. These results suggest that peritoneal macrophages express only low levels of SCR activity in situ and that posttranscriptional regulation after isolation leads to an increase in SCR activity that correlates with adherence of the macrophages in vitro.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Macrophages, Peritoneal Membrane Proteins Membrane Proteins Receptors, Immunologic Receptors, Lipoprotein Receptors, Lipoprotein Animals Animals Blotting, Northern Blotting, Northern Carbocyanines Carbocyanines Carbocyanines Cell Adhesion Cell Adhesion Cell Adhesion Cells, Cultured Cells, Cultured Cycloheximide Cycloheximide

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (40)

Cited by (13)

Source provenance

europepmc
last seen: 2026-10-14T06:08:39.101224+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-10-08T22:00:28.864438+00:00
License: CC0 · commercial use OK