Fresh mouse peritoneal macrophages have low scavenger receptor activity

J G Kim, Channa Keshava, C Keshava, A A Murphy, R E Pitas, Robert E. Pitas, S Parthasarathy, Srinivasan Parthasarathy
Journal of lipid research · 1997 · vol. 38(11) , pp. 2207–2215 · doi:10.1016/s0022-2275(20)34934-8 · PMID:9392418 · W2402457099
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Fresh mouse peritoneal macrophages exhibit low scavenger receptor activity that increases with in vitro culture due to posttranscriptional regulation, not changes in mRNA expression.

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Abstract

Peritoneal macrophages are easily isolated by lavage, suggesting that they are either nonadherent or weakly adherent in situ. Cultured macrophages express class A scavenger receptors (SCR), which mediate Ca2+-independent adhesion in vitro. We examined fresh peritoneal macrophages from mice and from women with endometriosis to determine whether the adherence of these cells was associated with increased expression of class A SCR. Fresh human macrophages were not immunoreactive to SCR antibodies; however, SCR immunoreactivity increased with time in culture. Fresh mouse and human macrophages took up minimal amounts of 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI)-acetyl-low density lipoproteins (Ac-LDL), a class A SCR ligand. Murine macrophages in culture for 24-72 h internalized four times more Ac-LDL than fresh cells. Cells cultured for 2 days incorporated 3.2 times more [14C] oleate than freshly isolated cells (55.7 +/- 7.9 versus 17.6 +/- 3.0 nmol/mg cell protein). In contrast to SCR activity, mouse macrophage SCR mRNA expression was similar in freshly isolated macrophages and those cultured for 3 days. These results suggest that peritoneal macrophages express only low levels of SCR activity in situ and that posttranscriptional regulation after isolation leads to an increase in SCR activity that correlates with adherence of the macrophages in vitro.

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Condition tags

endometriosis

MeSH descriptors

Macrophages, Peritoneal Membrane Proteins Receptors, Immunologic Receptors, Lipoprotein Animals Blotting, Northern Carbocyanines Carbocyanines Cell Adhesion Cell Adhesion Cells, Cultured Cycloheximide Cycloheximide Female Fluorescent Dyes Fluorescent Dyes Humans Lipoproteins, LDL Lipoproteins, LDL Macrophages, Peritoneal

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References (40)

Cited by (13)

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