Microvesicles released from ectopic endometrial foci as a potential biomarker of endometriosis

Magdalena Kajdos, Jacek Szymański, Jacek Szymanski, Hanna Jerczynska, Hanna Jerczyńska, Tomasz Stetkiewicz, Jacek R. Wilczyński, Jacek R Wilczynski
Ginekologia polska · 2022 · vol. 94(10) , pp. 780–791 · doi:10.5603/gp.a2022.0096 · PMID:36448351 · W4310464307
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Microvesicles positive for VEGF and MMP-9 were found in higher abundance in blood and peritoneal fluid of teratoma patients compared to endometriosis patients.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study investigated microvesicles (MVs) in peripheral blood and peritoneal fluid from 47 women undergoing surgery for benign ovarian lesions, comparing women with endometriosis (test group) to women with teratoma (control), while excluding participants with chronic cardiovascular, thyroid, or autoimmune diseases. Using an MV-isolation approach established during a preliminary phase, the authors assessed whether MVs were present and then aimed to determine whether they contained key angiogenesis mediators, specifically VEGF and metalloproteinase-9 (MMP-9), which have previously been implicated in endometriosis peritoneal fluid and lesions; a stated limitation is that the full results are not included in the provided text excerpt. This paper is centrally about endometriosis — it specifically evaluates microvesicles released from ectopic endometrial foci as a potential biomarker of endometriosis.

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Abstract

OBJECTIVES: Angiogenesis is engaged in endometriosis. It is regulated by regulatory factors and cytokines, transported in microvesicles. The purpose was to investigate the presence of MVs with vascular endothelial growth factor (VEGF) and metalloproteinase-9 (MMP-9) in peripheral blood and peritoneal fluid of women operated on for endometrioma or teratoma Material and methods:Microvesicles (MVs) were determined in blood samples and peritoneal fluid samples collected from women aged 20-60 years operated on for endometriosis (test group) and teratoma (control group). The final investigations were performed on 47 patients, who qualified for the study based on the meticulous inclusion criteria. MVs were analyzed by flow cytometry (FACS) using annexin V, antibodies for molecules characteristic of cells from endometriosis foci (keratin 18 (K18), CD105, CD146), and antibodies for intraepithelial vascular growth factor VEGF and metalloproteinase-9 (MMP-9). The sample was double "reading" using flow cytometry (FACSCantoII). RESULTS: Cytometry analysis confirmed MVs' presence in plasma and peritoneal fluid collected from patients with both endometriosis and teratomas. A statistically significant higher level of AnnexinV (+) MVs were observed in plasma samples of endometriosis patients. In the control group, there was a higher percentage of double-positive VEGF (+)/MMP-9 (+) and single MMP-9 (+) positive MVs in the serum. In the peritoneal fluid higher frequency of double-positive VEGF (+)/MMP-9 (+) MVs were found in the control group. However, the amount of VEGF (+) / MMP-9 (+) MVs object did not enable to differentiate between the test and control groups. The study was the first, in which MVs were confirmed in plasma and peritoneal fluid in benign adnexa tumors. CONCLUSIONS: Microvesicles are present in peripheral blood and peritoneal fluid samples collected from patients with endometriosis and teratomas. Microvesicles with proangiogenic factors (VEGF and MMP-9) are more abundant in blood and peritoneal fluid samples from patients with teratomas.

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Condition tags

endometriosisendometrioma

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

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