Ovarian Cancer Antigen CA 125 Enhances the Invasiveness of the Endometriotic Cell Line EEC 145

R Gaetje, Gaetje R, Winnekendonk DW, Dirk W. Winnekendonk, D W Winnekendonk, Anton Scharl, A Scharl, Scharl A, Manfred Kaufmann, Kaufmann M, M Kaufmann
Journal of the Society for Gynecologic Investigation · 1999 · vol. 6(5) , pp. 278–281 · doi:10.1177/107155769900600510 · PMID:10554768 · W2015709424
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This study investigated how ovarian cancer antigen CA 125 affects the invasiveness of the endometriotic cell line EEC 145.

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Abstract

ObjectiveOvarian cancer antigen CA 125 is used widely for diagnostic and therapeutic evaluation of ovarian cancer. Although the initial description of CA 125 was in 1981, its function is still unknown. In collagen gel system the invasiveness of the endometriotic cell line EEC 145 is enhanced by a proteinaceous factor in peritoneal fluid mimicking molecular features of CA 125. Therefore we hypothesize that this factor is CA 125.MethodsThe influence of heat-treated peritoneal fluid on the invasiveness of the endometriotic cell line EEC 145 and several human carcinoma cell lines (EJ28, RT112, AN3 CA, RL95-2, HEC-1-A, HeLa, MCF7, T-47D, and SK-OV-3) was investigated in a collagen gel invasion assay by addition of (1); 10% heat-treated peritoneal fluid, (2); peritoneal fluid after immunoprecipitation with antibodies directed to CA 125, and (3); purified CA 125.ResultsThe invasion index of the endometriotic cell line EEC 145 significantly increased from 4.78 +/- 0.98 to 6.57 +/- 1.58 (P = .0001) by addition of 10% heat-treated peritoneal fluid to the culture medium. The invasion-promoting effect of peritoneal fluid was abolished by reduction of the CA 125 concentration through immunoprecipitation and was mimicked in a dose-dependent manner by addition of CA 125 to the culture medium. The invasiveness of the investigated human carcinoma cell lines was not affected by heat-treated peritoneal fluid of CA 125.ConclusionThe results are consistent with the hypothesis that CA 125 can influence invasiveness in a benign endometriotic cell line.

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endometriosis

MeSH descriptors

CA-125 Antigen Endometriosis Ovarian Neoplasms Ascitic Fluid CA-125 Antigen Cell Line Collagen Endometriosis Endometriosis Female Gels Humans Ovarian Neoplasms Precipitin Tests

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