Evaluating the effect of conditioned medium from endometrial stem cells on endometriosis-derived endometrial stem cells

Seyedeh Saeideh Sahraei, Ali Kowsari, Faezeh Davoodi Asl, Mohsen Sheykhhasan, Leila Naserpoor, Leila Naserpour, Azar Sheikholeslami
Anatomy & cell biology · 2022 · vol. 55(1) , pp. 100–108 · doi:10.5115/acb.21.169 · PMID:35082175 · PMC8968229 · W4210340119
article OA: gold CC0 ⤵ 7 in-corpus citations
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Conditioned medium from healthy women's stem cells altered inflammatory and stemness gene expression in endometriosis-derived stem cells toward a normal state.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 ⓘ

This interventional experimental study examined whether conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women (NE-MenSCs) could alter endometriosis-derived MenSCs (E-MenSCs) from women with stage III–IV endometriosis. E-MenSCs were treated with NE-MenSCs-derived CM for 2, 4, or 6 days, after which surface markers were assessed by flow cytometry (including CD10 and selected CD markers) and inflammatory and stemness gene expression was measured by real-time PCR. The authors report that E-MenSCs showed morphology changes toward a more “normal” phenotype in the presence of CM, with a significant decrease in CD10, and that IL-1α, COX-2, and HIF-1α and the stemness genes OCT4, NANOG, and SOX2 had significantly different expression levels after CM exposure. The study’s main limitation is the very small sample size (n=3 per group) from which MenSCs were derived. This paper is centrally about endometriosis—specifically testing whether NE-MenSCs conditioned medium can normalize inflammatory and stemness characteristics of endometriosis-derived MenSCs.

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Abstract

Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Endometriosis-derived MenSCs (E-MenSCs) were treated with CM derived from healthy women's MenSCs (non-endometriosis derived MenSCs [NE-MenSCs]). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the interleukin-1, cyclooxygenase-2, and hypoxia-inducible factor 1α as inflamaturay genes and octamer-binding transcription factor 4, NANOG, and sex determining region Y-box 2 as stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NEMenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

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endometriosis

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