Endometriosis and regional lymph node involvement in a rat model

Zusammenfassung ZIELSETZUNGEN: Zielsetzung dieser Arbeit war die Etablierung eines experimentellen Tiermodells für Darmendometriose in der Ratte mit spezieller Berücksichtigung der Ausbreitung von Endometriose in regionale Lymphknoten. METHODIK: An Sprague-Dawley Ratten wurde eine autologe Transplantation von Endometrium vom Uterus in die Ileocoecalregion durchgeführt. Nach zwei Monaten wurden Endometrioseläsionen am Darm sowie regionale mesenterische Lymphknoten chirurgisch entfernt und histologisch untersucht. Zielparameter war der Nachweis von Endometrioseherden im Ileococum und lymphogen verschleppten Endometrioseherden in den mesenterialen Lymphknoten. ERGEBNISSE: Bei 20 Ratten wurde die Transplantation durchgeführt. Endometrioseherde in der Ileocoecalregion wurden nach zwei Monaten in 19/20 (95 %) Tieren nachgewiesen. Ein mesenterialer Lymphknoten wurde in 18/20 Tieren identifiziert und reseziert. Endometrioseherde in mesenterialen Lymphknoten wurden in keinem der untersuchten 18 Lymphknoten nachgewiesen. SCHLUSSFOLGERUNGEN: Experimentelle Darmendometriose kann in einem Rattenmodell durch autologe Transplantation von Endometrium vom Uterus in die Ileocoecalregion erfolgreich induziert werden. In diesem experimentellen Modell konnte keine regionale Verschleppung von Endometrioseherden in regionale Lymphknoten nachgewiesen werden. Summary OBJECTIVE(S): To establish an experimental bowel endometriosis model in the rat and to investigate regional lymph node involvement. STUDY DESIGN: Twenty Sprague-Dawley rats were subjected to autologous transplantation of endometrial tissue to the ileocecum by laparotomy. After two months, endometriotic lesions and mesenteric lymph nodes were harvested by repeat laparotomy subjected to histologic assessment regarding the histologically verified presence of endometriosis in the ileocecum and in mesenteric lymph nodes. 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