{"paper_id":"dc7de8bf-4d01-488e-bf68-713eb87a6a7a","body_text":"Zusammenfassung\nZIELSETZUNGEN: Zielsetzung dieser Arbeit war die Etablierung eines experimentellen Tiermodells für Darmendometriose in der Ratte mit spezieller Berücksichtigung der Ausbreitung von Endometriose in regionale Lymphknoten. METHODIK: An Sprague-Dawley Ratten wurde eine autologe Transplantation von Endometrium vom Uterus in die Ileocoecalregion durchgeführt. Nach zwei Monaten wurden Endometrioseläsionen am Darm sowie regionale mesenterische Lymphknoten chirurgisch entfernt und histologisch untersucht. Zielparameter war der Nachweis von Endometrioseherden im Ileococum und lymphogen verschleppten Endometrioseherden in den mesenterialen Lymphknoten. ERGEBNISSE: Bei 20 Ratten wurde die Transplantation durchgeführt. Endometrioseherde in der Ileocoecalregion wurden nach zwei Monaten in 19/20 (95 %) Tieren nachgewiesen. Ein mesenterialer Lymphknoten wurde in 18/20 Tieren identifiziert und reseziert. Endometrioseherde in mesenterialen Lymphknoten wurden in keinem der untersuchten 18 Lymphknoten nachgewiesen. SCHLUSSFOLGERUNGEN: Experimentelle Darmendometriose kann in einem Rattenmodell durch autologe Transplantation von Endometrium vom Uterus in die Ileocoecalregion erfolgreich induziert werden. In diesem experimentellen Modell konnte keine regionale Verschleppung von Endometrioseherden in regionale Lymphknoten nachgewiesen werden.\nSummary\nOBJECTIVE(S): To establish an experimental bowel endometriosis model in the rat and to investigate regional lymph node involvement. STUDY DESIGN: Twenty Sprague-Dawley rats were subjected to autologous transplantation of endometrial tissue to the ileocecum by laparotomy. After two months, endometriotic lesions and mesenteric lymph nodes were harvested by repeat laparotomy subjected to histologic assessment regarding the histologically verified presence of endometriosis in the ileocecum and in mesenteric lymph nodes. RESULT(S): A total of 20 rats were subjected to autologous transplantation of endometrial tissue to the ileocecum. Ileocecal endometriosis was successfully induced in 19/20 (95%) rats. In 18/20 animals, the mesenteric lymph node was identified and resected. Regional lymph node involvement by endometriosis was identified in 0/18 mesenteric lymph nodes. CONCLUSION(S): Endometriosis can be successfully induced in a rat model by autologous transplantation of endometrial tissue. In this experimental rodent endometriosis model, regional mesenteric lymph node involvement by endometriosis was not identified.\nSimilar content being viewed by others\nReferences\nBerkley KJ, Rapkin AJ, Papka RE. The pains of endometriosis. Science 2005;308:1587–9\nOlive DL, Schwartz LB. Endometriosis. N Engl J Med 1993;328:1759–69\nBallouk F, Ross JS, Wolf BC. Ovarian endometriotic cysts an analysis of cytologic atypia and DNA ploidy patterns. Am J Clin Pathol 1994;102:415–9\nJimbo H, Hitomi Y, Yoshikawa H, Yano T, Momoeda M, Sakamoto. Evidence for monoclonal expansion of epithelial cells in ovarian endometrial cysts. Am J Pathol 1997;150:1173–8\nThomas EJ, Campbell IG. Molecular genetic defects in endometriosis. Gynecol Obstet Invest 2000;50(Suppl.):44–50\nNezhat F, Cohen C, Rahaman J, Gretz H, Cole P, Kalir T. Comparative immunohistochemical studies of bcl-2 and p53 proteins in benign and malignant ovarian endometriotic cysts. Cancer 2002;1:2935–40\nLorente-Poyatos R, Palacios-Perez A, Bravo-Bravo F, Lopez-Caballero FJ, Bouhmidi A, Huertas-Nadal C, Ruiz-Escolano R. Rectosigmoid endometriosis with lymph node involvement. Gastroenterol Hepatol 2003;26:23–5\nInsabato L, Pettinato G. Endometriosis of the bowel with lymph node involvement. A report of three cases and review of the literature. Pathol Res Pract 1996;192(9):957–61\nThomakos N, Rodolakis A, Vlachos G, Papaspirou I, Markaki S, Antsaklis A. A rare case of rectovaginal endometriosis with lymph node involvement. Gynecol Obstet Invest 2006;62(1):45–7\nAbrao MS, Podgaec S, Dias JA Jr, Averbach M, Garry R, Ferraz Silva LF, Carvalho FM. Deeply infiltrating endometriosis affecting the rectum and lymph nodes. Fertil Steril 2006;86(3):543–7\nNoël JC, Chapron C, Fayt I, Anaf V. Lymph node involvement and lymphovascular invasion in deep infiltrating rectosigmoid endometriosis. Fertil Steril 2007 Aug 4; [Epub ahead of print]\nMechsner S, Weichbrodt M, Riedlinger WF, Bartley J, Kaufmann AM, Schneider A, Köhler C. Estrogen and progestogen receptor positive endometriotic lesions and disseminated cells in pelvic sentinel lymph nodes of patients with deep infiltrating rectovaginal endometriosis: a pilot study. Hum Reprod 2009;23(10):2202–9\nHuhn FO. \"Glandular inclusions\" in the pelvic lymph nodes and endometriosis. Geburtshilfe Frauenheilkd 1962;22:335–46\nLange P. Endometriosis-like formations in the lymph nodes of the pelvic wall. Acta Obstet Gynecol Scand 1955;34:111–9\nRegidor-Brandau PA, Pfaffenbach B, Metz KA, Schindler AE. Endometriosis in retroperitoneal lymph nodes. Geburtshilfe Frauenheilkd 1994;54:372–4\nJavert CT. Pathogenesis of endometriosis based on endometrial homeoplasia, direct extension, exfoliation and implantation, lymphatic and hematogenous metastasis, including five case reports of endometrial tissue in pelvic lymph nodes. Cancer 1949;2:399–410\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nGong, Y., Hong, L., Zheng, CC. et al. Endometriosis and regional lymph node involvement in a rat model. Wien Klin Wochenschr 123, 432–435 (2011). https://doi.org/10.1007/s00508-011-0003-2\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s00508-011-0003-2","source_license":"CC0","license_restricted":false}