NeiyiKangfu tablets control the progression of endometriosis through inhibiting RAF/MEK/ERK signal pathway by targeting RKIP

Yi Wen, Ling-Xiu Fan, Lingxiu Fan, Lili Pang, Ting-Ting Zhao, Tingting Zhao, Ruonan Li, Ying Zhang, Liye Zhang, Wei Yang
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology · 2022 · vol. 38(12) , pp. 1136–1146 · doi:10.1080/09513590.2022.2162495 · PMID:36592742 · W4313454765
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Abstract

BACKGROUND: NeiyiKangfu tablets (NYKF) are widely used clinically for the treatment of endometriosis (EMS), whose mechanism of action has been extensively studied. Researchers have found that NYKF may control the development of ectopic lesions by inhibiting angiogenesis and inflammatory cytokine secretion. Nevertheless, NYKF's mechanism of action remains unclear. METHODS: experiments. EMS model mice were treated with NYKF and the pro-inflammatory factors and apoptosis of ectopic endometrium as well as RAF/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling activation were assessed. In addition, human endometriosis-derived immortalized entopic stromal (hEM15A) cells transfected with or without RAF kinase inhibitor protein (RKIP)-small-interfering RNA (siRNA) were also treated with NYKF and the proliferation, migration, apoptosis, and RAF/MEK/ERK signaling activation were measured by Cell Counting Kit-8 (CCK-8), flow cytometry, Transwell, and western blot, respectively. RESULTS: Results showed that NYKF increased the expression of RKIP, inhibited RAF/MEK/ERK signaling activation, and induced apoptosis while inhibiting proliferation and migration both in EMS mice and hEM15A cells. RKIP knockdown could inhibit the effect of NYKF treatment, leading to the activation of RAF/MEK/ERK signaling and the proliferation and migration of hEM15A cells. CONCLUSIONS: In conclusion, these results suggest that NYKF treatment promotes apoptosis and inhibits proliferation and migration in EMS by inhibiting the RAF/MEK/ERK signaling pathway by targeting RKIP.

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Condition tags

endometriosis

MeSH descriptors

Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Drugs, Chinese Herbal Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

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