Experimental study of influence to hemangiogenic factors of Neiyikangfu Pills on in utero and ectopic endometrium

Objective To discover the mechanism of Neiyikangfu Tablets in treating endometriosis by studying the influence to hemangiogenic factors on rats' in utero and ectopic endometrium. Methods Established endometriosis models of rats and distributed them randomly into high dose and medium dose group of Neiyikangfu Tablets,Danazol group,Danefukang condensed decoction group,model group and blank group. Dyeing with immuno-histochemical SP,semiquantitatively detected the expression of VEGF and ENS in utero and ectopic endometrium,and signed ectopic endometrial vascular endotheliocyte by CD31 to detect MVD.Results The expression of VEGF,V/E,MVD and number of micro vascular of ectopic endometrium was low and ENS was high in high and medium dose Neiyikangfu Tablets groups (P0.01).But there was no difference in V/E,MVD and number of micro vascular between in utero endometrium group and blank group(P0.05). Conclusions Maybe Neiyikangfu Tablets can inhibit the angiogenesis process of ectopic endometrium and improve the extinction of ectopic endometrium,help keeping the balance of stimulating and inhibiting factors of angiogenesis,but can't inhibit the physiologic angiogenesis process of in utero endometrium. It can reduce MVD of ectopic endometrium maybe by inhibiting the angiogenesis of ectopic endometrium.