Disturbed progesterone signalling in an advanced preclinical model of endometriosis

Esfandiari F, Fereshteh Esfandiari, Heidar Heidari Khoei, Heidari Khoei H, Saber M, Maryam Saber, Favaedi R, Raha Favaedi, Abbas Piryaei, Moini A, Ashraf Moini, Shahhoseini M, Maryam Shahhoseini, Fariba Ramezanali, Ramezanali F, Firouzeh Ghaffari, Ghaffari F, Hossein Baharvand
Reproductive biomedicine online · 2021 · vol. 43(1) , pp. 139–147 · doi:10.1016/j.rbmo.2020.12.011 · PMID:34049811 · W3137007212
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This study investigated disturbed progesterone signaling in an advanced preclinical model of endometriosis to elucidate its role in the disease's progression.

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Abstract

Research questionDo human endometriosis organoids recapitulate aberrant progesterone signalling in the disease to serve as advanced experimental models for uncovering epigenetic mechanisms involved in attenuated progesterone response in endometriosis?DesignInitially, the organoids were established from acquired biopsies (women with and without endometriosis) and characterized by morphological, histological and immunostaining analyses.ResultsA panel of endometriosis-related genes showed a pattern of expressions in cytochrome c oxidase subunit II (COX2), matrix metalloproteinase 2 (MMP2), MMP9, tissue inhibitor of metalloproteinase-3 (TIMP3), transforming growth factor beta 1 (TGF-β1), and zinc finger E-box binding homeobox 1 (ZEB1), and a contradictory expression pattern for cadherin (CDH1), POU class 5 homeobox 1 (POU5F1; also known as OCT4), and Nanog homeobox (NANOG) in the endometriosis organoids that is concordant with published research. These endometriosis organoids failed to upregulate 17β-Hydroxysteroid dehydrogenase 2 (17HSDβ2), progestogen associated endometrial protein (PAEP), secreted phosphoprotein 1 (SPP1), and leukaemia inhibitory factor (LIF) in response to progesterone at the level observed in control endometrium organoids. Progesterone receptor B (PRB) gene expression significantly decreased in both eutopic and ectopic organoids compared with control endometrium organoids. DNA hypermethylation, as an epigenetic mechanism for suppression of transcription, was detected at the PRB promoter in the eutopic, but not ectopic, organoids. Therefore, other epigenetic mechanisms, such as histone modifications and microRNAs, may be responsible for PRB downregulation in ectopic organoids.ConclusionsEndometriosis organoids are powerful preclinical models that can be used to investigate the molecular mechanisms involved in endometriosis-associated progesterone resistance.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Organoids Progesterone Receptors, Progesterone Adult DNA Methylation Endometriosis Female Humans Organoids Progesterone Receptors, Progesterone

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