Identification of FZD7 as a potential ferroptosis-related diagnostic gene in endometriosis by bioinformatics analysis

Jianyun Huang, Jinbo Li, Xiao Li, Hongling Guo, Shuqin Chen
Scientific reports · 2025 · vol. 15(1) , pp. 7172 · doi:10.1038/s41598-025-90803-9 · PMID:40021920 · W4408036615
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

Bioinformatics analysis identified FZD7 as a ferroptosis-related gene upregulated in endometriosis, correlating with M2 macrophage infiltration and showing diagnostic potential.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-10 ⓘ

This study used bioinformatics and validation experiments to identify ferroptosis-related diagnostic genes in endometriosis (EMS). Differentially expressed ferroptosis-related genes were screened across multiple GEO microarray datasets comparing 12 women with EMS to 10 control patients without endometriosis, and only FZD7 consistently emerged as differentially expressed across five transcriptome datasets, with high diagnostic performance by ROC analysis (AUC up to 100% in one dataset). The authors also reported that CIBERSORT-inferred immune infiltration showed significantly increased macrophages, especially M2 macrophages, in EMS, and that FZD7 correlated positively with M2 macrophage infiltration and was upregulated in endometrial stromal cells co-cultured with macrophages; RT-qPCR and Western blot validated FZD7 upregulation in ectopic lesions. The study’s main caveat is that it relies on cross-dataset bioinformatics associations from relatively small patient cohorts (12 EMS cases, 10 controls) before drawing mechanistic conclusions. This paper is centrally about endometriosis — it identifies FZD7 as a potential ferroptosis-related diagnostic gene and examines its association with macrophage infiltration in EMS.

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Abstract

An increasing number of research have suggested that ferroptosis plays an important role in endometriosis (EMS). This study was to identify a ferroptosis-related diagnosis gene in EMS by using bioinformatics. R Bioconductor package limma was used to analyzed the differentially expressed genes (DEGs) between the EMS groups and control groups. CIBERSORT was used to analyze the differences between the EMS group and control group of 22 immune cells. Quantitative real-time PCR (RT-qPCR) and Western blot (WB) were used to validate the expression level of FZD7 in tissue samples. The study found that FZD7 was upregulated and showed good diagnostic value in five EMS transcriptome databases. RT-qPCR and WB experiments also verified that FZD7 was upregulated in EMS. Moreover, we found that macrophages, especially M2 macrophages, were significantly infiltrated in EMS. FZD7 was positively correlated with M2 macrophage infiltration, and was up-regulated in the endometrial stromal cells co-cultured with macrophages. The study identified an ferroptosis repressor gene, FZD7, validated in five EMS transcriptome datasets, which is significantly up-regulated in ectopic lesions of EMS and is a potential target for the treatment of EMS.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Ferroptosis Ferroptosis Ferroptosis Ferroptosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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