RNA-sequencing identifies differentially expressed genes in T helper 17 cells in peritoneal fluid of patients with endometriosis

Yanping Jiang, Jiang YP, Yan-ping Jiang, Ya-Qin Peng, Yaqin Peng, Peng YQ, Lu Wang, Wang L, Jian Qin, Qin J, Zhang Y, Yan Zhang, Yu-Zi Zhao, Yuzi Zhao, Zhao YZ, Tan AL, Aili Tan, Ai-Li Tan, Wang SJ, Shujun Wang, Shu-Jun Wang, Pi J, Jie Pi
Journal of Reproductive Immunology · 2021 · vol. 149 , pp. 103453 · doi:10.1016/j.jri.2021.103453 · PMID:34839179 · W3213851123
article OA: closed CC0 ⤵ 3 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This RNA-sequencing study identified genes with altered expression levels in T helper 17 cells isolated from the peritoneal fluid of women diagnosed with endometriosis.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Innate and adaptive immune factors play significant roles in the pathophysiology of endometriosis. T helper 17 (Th17) cells, a pro-inflammatory T cell subset, were considered to contribute to the progression of endometriosis lesions. However, the regulatory mechanisms of Th17 cells in endometriosis remain unidentified, partially due to the difficulty in recovering live Th17 cells from endometriosis patients. In this study, by flow cytometry analysis of a set of chemokine receptors including CXCR3, CCR4, CCR10, and CCR6, live RORγt-and-IL-17A-expressing Th17 cells were enriched from peritoneal fluid (PF) of patients with different stages of endometriosis for the first time, RNA-sequencing (RNA-Seq) of these PF Th17 cells revealed significantly up-regulated genes and down-regulated genes in stage I-II and stage III-IV endometriosis, compared with their counterparts in normal PF. In conclusion, this study provides a novel method to isolate live Th17 cells from endometriosis patients, unveils an array of differentially expressed genes in endometriosis Th17 cells, and offers valuable gene expression profile information for endometriosis clinical research.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Endometriosis Th17 Cells Adult Ascitic Fluid Endometriosis Female Gene Expression Regulation Humans Interleukin-17 Interleukin-17 Nuclear Receptor Subfamily 1, Group F, Member 3 Nuclear Receptor Subfamily 1, Group F, Member 3 Receptors, Chemokine Receptors, Chemokine Receptors, CXCR3 Receptors, CXCR3 Sequence Analysis, RNA Th17 Cells

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (22)

Cited by (3)

Source provenance

europepmc
last seen: 2026-07-31T06:09:14.520117+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:24:08.918168+00:00
License: CC0 · commercial use OK