Hsa_circ_0063526 promotes the development of endometriosis by sponging miRNA-141-5p

Zhangming Wei, Mengmeng Zhang, Xinyue Zhang, Xiaoling Fang, Liping Li
In: Research Square · 2021 · doi:10.21203/rs.3.rs-249032/v1 · W3134954494
preprint OA: green CC0 ⤵ 1 in-corpus citation
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Hsa_circ_0063526 expression is elevated in endometriosis and promotes lesion development by sponging miRNA-141-5p, inhibiting cell invasion, metastasis, and proliferation.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This preprint studied whether the circular RNA hsa_circ_0063526 (circ-RanGAP1) regulates endometriosis development by interacting with microRNA-141-5p. Using RT-qPCR on ectopic endometriosis tissues from 31 women and control endometrium from 10 women, the authors reported higher hsa_circ_0063526 in ectopic lesions and a negative correlation between hsa_circ_0063526 and miRNA-141-5p; in End1/E6E7 cells, hsa_circ_0063526 knockdown reduced invasion, metastasis-related behaviors, and proliferation, while miR-141-5p inhibition rescued these effects. In an endometriosis mouse model, si-hsa_circ_0063526 and miR-141-5p–related interventions reportedly reduced lesion size and altered endometriosis gene expression, but the paper does not clearly state limitations beyond noting it is a non–peer reviewed preprint. This paper is centrally about endometriosis — it proposes that hsa_circ_0063526 promotes endometriosis by sponging miRNA-141-5p.

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Abstract

Abstract Background:Endometriosis can cause various social burdens. Abnormal circular RNAs level lead to changes of related gene expression, thereby mediating the occurrence and development of a series of diseases. The research of circRNA in endometriosis is still in its infancy. This study will explore the role of hsa_circ_0063526 with microRNA-141-5p in the development of endometriosis.Methods:The expression of genes was detected by RT-qPCR. Transwell, wound-healing, EdU assay were performed on endometriosis patient’s End1 / E6E7 cell. PCR and immunohistochemistry were used to detect the expression of candidate regulatory genes in ectopic lesions in endometriosis mice model.Results:The expression level of hsa_circ_0063526 in endometriosis patients’ ectopic tissue is higher than control(P <0.05), The expression levels of hsa_circ_0063526 and miRNA-141-5P in ectopic tissue of endometriosis were negatively correlated (P < 0.05).Knockdown hsa_circ_0063526 inhibited the invasion, metastasis, and proliferation ability of End1 / E6E7 cell. The inhibition of microRNA-141-5p can rescue this inhibitory effect (P <0.05). In-vivo experiments showed miR-141-5p and si-hsa_circ_0063526 treatment reduced the lesion size and regulated endometriosis genes.Conclusion:Hsa_circ_0063526 is probably involved in regulating the occurrence and development of endometriosis by sponging miR-141-5p. hsa_circ_0063526 and miR-141-5p are possible to become biomarkers and therapeutic targets for endometriosis.

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endometriosis

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