microRNA-141 inhibits TGF-β1-induced epithelial-to-mesenchymal transition through inhibition of the TGF-β1/SMAD2 signalling pathway in endometriosis

Sixue Wang, Xiaoling Fang, Mengmeng Zhang, Tingting Zhang, Juan Deng, Xiaomeng Xia
Archives of gynecology and obstetrics · 2020 · vol. 301(3) , pp. 707–714 · doi:10.1007/s00404-019-05429-w · PMID:31903498 · W2997989869
article OA: hybrid CC0 ⤵ 11 in-corpus citations
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This study found that microRNA-141 inhibits TGF-β1-induced epithelial-to-mesenchymal transition and proliferation in endometriosis by targeting the TGF-β1/SMAD2 signaling pathway.

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Abstract

PURPOSE: Recent studies have demonstrated the differential expression of micro(mi)RNAs in endometriosis. Previously, we reported the low expression of miR-141 in patients with this disease. Epithelial-to-mesenchymal transition (EMT) and the transforming growth factor-beta1 (TGF-β1)-induced SMAD2 signalling pathway are central to tumour proliferation and invasion. However, the role of miR-141 in regulating the TGF-β1/SMAD2 signalling pathway and the associated EMT to be elucidated. METHODS: The levels of TGF-β1/SMAD2 signalling and EMT markers expression in eutopic and ectopic endometria of endometriosis were determined by immunohistochemistry and western blot analyses. MiR-141 expression was analysed by quantitative reverse-transcription polymerase chain reaction. Cellular invasion and proliferation were determined by transwell and CCK-8 assays, respectively. Functional assay of miR-141 was performed using plasmid and shRNA transfection methods. RESULT: The presence of miR-141, EMT, and TGF-β1/SMAD2 signalling markers were detected in eutopic and ectopic endometria of endometriosis. TGF-β1-induced EMT in Ishikawa (ISK) cells by activating the SMAD2 signalling pathway, whereas miR-141 inhibited the TGF-β1-induced EMT, proliferation and invasion abilities of these cells. CONCLUSION: These data identify miR-141 as a novel driver of EMT in endometriosis, implicates the link between miR-141 and TGF-β1/SMAD2 signalling pathway in the context of endometriosis, and underscore the role of EMT in the development of endometriosis.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Endometriosis Epithelial-Mesenchymal Transition MicroRNAs Transforming Growth Factor beta1 Endometriosis Endometriosis Epithelial-Mesenchymal Transition Female Humans MicroRNAs MicroRNAs Signal Transduction Transfection Transforming Growth Factor beta1

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