Immunohistochemical expression of Drosha is reduced in eutopic and ectopic endometrium of women with adenomyosis

I. Ormenezi, Alfredo Ribeiro‐Silva, Júlio César Rosa e Silva, Juliana Meola, Francisco José Cândido dos Reis, Omero Benedicto Poli‐Neto
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas · 2022 · vol. 55 , pp. e12375 · doi:10.1590/1414-431x2022e12375 · PMID:36515351 · W4312065682
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Drosha expression was reduced in both eutopic and ectopic endometrium of women with adenomyosis compared to controls, with no significant changes in Dicer or Exportin-5.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study evaluated immunohistochemical expression of the miRNA-processing proteins Dicer, Drosha, and Exportin-5 in paired eutopic and ectopic endometrium from 22 women with adenomyosis, compared with eutopic endometrium from 10 control hysterectomy patients without adenomyosis, leiomyoma, or endometriosis. After automated slide scanning, Drosha expression was significantly lower in women with adenomyosis in both eutopic endometrium (69.9±3.4% vs 85.2±2.9%) and ectopic endometrium (59.6±3.2% vs 69.9±3.4%), while no significant differences were found for Dicer or Exportin-5. Drosha expression in ectopic tissue correlated with paired eutopic tissue within the adenomyosis group. This paper is centrally about adenomyosis — it links reduced Drosha protein expression in eutopic and ectopic endometrium to the miRNA biogenesis pathway.

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Abstract

The objective of this study was to evaluate the immunohistochemical expression of Dicer, Drosha, and Exportin-5 in the eutopic and ectopic endometrium of women with adenomyosis. Twenty-two paired ectopic and eutopic endometrium from women with adenomyosis and 10 eutopic endometrium samples from control women undergoing hysterectomy were included in the study. Paraffin-embedded tissue blocks were cut and stained for immunohistochemistry. The percentage of epithelial cells positively marked was identified digitally after an automated slide scanning process. Mann-Whitney test or Wilcoxon signed-rank test was performed for independent and paired groups, respectively. A lower expression of Drosha was observed in the eutopic endometrium of women with adenomyosis than in the eutopic endometrium of women without the disease (69.9±3.4% vs 85.2±2.9%, respectively) (P=0.016; 95%CI: 3.4 to 27.4%). We also detected lower Drosha expression in the ectopic endometrium of women with adenomyosis than in the eutopic endometrium of the same women (59.6±3.2% vs 69.9±3.4%, respectively) (P=0.004; 95%CI: 2.3 to 16.7%). Additionally, we observed a correlation between Drosha expression in the ectopic and paired eutopic endometrium (P=0.034, rho=0.454). No significant difference in Dicer or Exportin expression was observed. Predominant pattern of cytoplasmic staining for the anti-Drosha antibody and both a nuclear and cytoplasmic pattern for the anti-Exportin antibody were observed. Drosha expression was significantly lower in the endometrium of women with adenomyosis compared to the eutopic endometrium of asymptomatic women without the disease. Furthermore, its expression was lower in the ectopic endometrium but correlated to the paired eutopic endometrium.

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Condition tags

mesh:D004715adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

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